Anti-Synphilin 1 antibody (ab6179)
Key features and details
- Rabbit polyclonal to Synphilin 1
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-Synphilin 1 antibody
See all Synphilin 1 primary antibodies -
Description
Rabbit polyclonal to Synphilin 1 -
Host species
Rabbit -
Specificity
Immunohistochemical analysis of human brain indicates a high level of specificity for this antiserum. Cross reactivity: This antibody is known to react with rat and human. Cross reactivity with other species has not been tested yet. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide:
CASKGKNKAA
conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 911-919 of Human Synphilin 1. -
General notes
This antibody stains Lewy bodies in Dorsal raphe nucleus of Parkinson’s diseased human brain.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7 -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
Target
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Function
Isoform 2 inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. Isoform 2 inhibits autoubiquitination and proteasomal degradation of SIAH1, and thereby increases cellular levels of SIAH. Isoform 2 modulates SNCA monoubiquitination by SIAH1. -
Tissue specificity
Detected in brain (at protein level). Widely expressed, with highest levels in brain, heart and placenta. -
Involvement in disease
Parkinson disease -
Sequence similarities
Contains 6 ANK repeats. -
Post-translational
modificationsUbiquitinated; mediated by SIAH1, SIAH2 or RNF19A and leading to its subsequent proteasomal degradation. In the absence of proteasomal degradation, ubiquitinated SNCAIP accumulates in cytoplasmic inclusion bodies. Isoform 2 is subject to limited ubiquitination that does not lead to proteasomal degradation. -
Cellular localization
Cytoplasm. Detected in cytoplasmic inclusion bodies, together with SNCA. - Information by UniProt
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Database links
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Alternative names
- Alpha synuclein interacting protein antibody
- Alpha-synuclein-interacting protein antibody
- MGC39814 antibody
see all
Images
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IHC image of ab6179 staining in rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6179, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
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Datasheet download
References (3)
ab6179 has been referenced in 3 publications.
- Liang X et al. Panaxadiol inhibits synaptic dysfunction in Alzheimer's disease and targets the Fyn protein in APP/PS1 mice and APP-SH-SY5Y cells. Life Sci 221:35-46 (2019). PubMed: 30735733
- Kühn K et al. Parkin expression in the developing mouse. Brain Res Dev Brain Res 149:131-42 (2004). PubMed: 15063093
- Specht CG & Schoepfer R Deletion of the alpha-synuclein locus in a subpopulation of C57BL/6J inbred mice. BMC Neurosci 2:11 (2001). PubMed: 11591219