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Products:Neuroscience >> Neurotransmitter >> Transporters >> Other
Overview
Product name
Anti-Syntaxin 1A + Syntaxin 1B2 antibody
Description
Goat polyclonal to Syntaxin 1A + Syntaxin 1B2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Sheep, Chicken, Cow, Human, Zebrafish
Immunogen
Synthetic peptide: KDRTQELRTAKD, corresponding to N terminal amino acids 2/13 of Human Syntaxin 1A + Syntaxin 1B2
KDRTQELRTA KD
Positive control
Mouse Brain lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Neuroscience >> Neurotransmitter >> Transporters >> Other
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Western blot - Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab39986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 35 kDa. (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Syntaxins are potentially involved in the docking of synaptic vesicles at presynaptic active zones and may play a key role in neurotransmitter exocytosis. Three isoforms are produced by alternative splicing. Isoform 1 is expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain. Syntaxin 1A interacts with VAPA and SYBU and is a member of the SNARE core complex containing SNAP25 and VAMP2. In addition, it is found in complexes with SNAP25, SNAP23 and VAMP8. Haploinsufficiency of STX1A may result in particular cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome, a rare developmental disorder.
Cellular localization
Cell membrane; single-pass type IV membrane protein; Isoform 2 is thought to be secreted
Alternative names
- adipocyte C1Q and collagen domain containing antibody
- adiponectin antibody
- adipose most abundant gene transcript 1 antibody
see all
Database links
- Entrez Gene: 282377 Cow
- Entrez Gene: 112755 Human
- Entrez Gene: 6804 Human
- Entrez Gene: 56216 Mouse
- Entrez Gene: 24923 Rat
- Omim: 186590 Human
- SwissProt: P61267 Cow
- SwissProt: P61266 Human
see all
Anti-Syntaxin 1A + Syntaxin 1B2 antibody images:
Western blot - Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)
Anti-Syntaxin 1A + Syntaxin 1B2 antibody (ab39986) at 0.003 µg/ml (for 1 hour) + Mouse Brain lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
References for Anti-Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)
ab39986 has not yet been referenced specifically in any publications.
Publishing research using ab39986? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"