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Western blot - Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)
Western blot - Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)
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Product Name 

Syntaxin 1A + Syntaxin 1B2 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to Syntaxin 1A + Syntaxin 1B2

Immunogen 

Synthetic peptide: KDRTQELRTAKD, corresponding to N terminal amino acids 2/13 of Human Syntaxin 1A + Syntaxin 1B2
BLAST 'KDRTQELRTAKD' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Ms

Predicted to react 
(species key)

Hu, Rat, Chk, Cow, Shp, Zfsh
(Due to sequence homology)

Tested applications 
(see key)

ELISA, WB


Abreviews 

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 35 kDa. (predicted molecular weight: 33 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse Brain lysate

Cellular localization 

Cell membrane; single-pass type IV membrane protein; Isoform 2 is thought to be secreted

Research areas 

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Neuroscience >> Neurotransmitter >> Transporters >> Other

Relevance 

Syntaxins are potentially involved in the docking of synaptic vesicles at presynaptic active zones and may play a key role in neurotransmitter exocytosis. Three isoforms are produced by alternative splicing.
Isoform 1 is expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
Syntaxin 1A interacts with VAPA and SYBU and is a member of the SNARE core complex containing SNAP25 and VAMP2. In addition, it is found in complexes with SNAP25, SNAP23 and VAMP8.
Haploinsufficiency of STX1A may result in particular cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome, a rare developmental disorder.

 

Alternative names Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

282377    (Cow)
6804    (Human)
112755    (Human)
56216    (Mouse)
24923    (Rat)

Omim

    

186590    (Human)

SwissProt

    

P61267    (Cow)
P61266    (Human)
P61264    (Mouse)
P61265    (Rat)
P61268    (Sheep)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Syntaxin 1A + Syntaxin 1B2 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Syntaxin 1A + Syntaxin 1B2 antibody images, references, products related to ab39986 and other tools.

 

Syntaxin 1A + Syntaxin 1B2 antibody images:

Product image  Western blot - Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)

Western blot - Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)

Syntaxin 1A + Syntaxin 1B2 antibody (ab39986) at 0.003 µg/ml (for 1 hour) + Mouse Brain lysate in RIPA buffer at 35 µg
developed using the ECL technique

Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to Syntaxin 1A + Syntaxin 1B2

 

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Lysates

 

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