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Anti-Syntaxin 1A + Syntaxin 1B2 antibody
Goat polyclonal to Syntaxin 1A + Syntaxin 1B2
WB, ELISAmore details
Reacts with
Mouse
Predicted to work with
Rat, Sheep, Chicken, Cow, Human, Zebrafish
Synthetic peptide: KDRTQELRTAKD, corresponding to N terminal amino acids 2/13 of Human Syntaxin 1A + Syntaxin 1B2
KDRTQELRTA KD
Mouse Brain lysate
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Polyclonal
IgG
Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Neuroscience >> Neurotransmitter >> Transporters >> Other
Western blot - Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)
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Our Abpromise guarantee covers the use of ab39986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:32,000
WB: Use at a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 35 kDa. (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Syntaxins are potentially involved in the docking of synaptic vesicles at presynaptic active zones and may play a key role in neurotransmitter exocytosis. Three isoforms are produced by alternative splicing. Isoform 1 is expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain. Syntaxin 1A interacts with VAPA and SYBU and is a member of the SNARE core complex containing SNAP25 and VAMP2. In addition, it is found in complexes with SNAP25, SNAP23 and VAMP8. Haploinsufficiency of STX1A may result in particular cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome, a rare developmental disorder.
Cell membrane; single-pass type IV membrane protein; Isoform 2 is thought to be secreted
Western blot - Syntaxin 1A + Syntaxin 1B2 antibody (ab39986)

Anti-Syntaxin 1A + Syntaxin 1B2 antibody (ab39986) at 0.003 µg/ml (for 1 hour) + Mouse Brain lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
ab39986 has not yet been referenced specifically in any publications.
Publishing research using ab39986? Please let us know so that we can cite the reference in this datasheet
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Anti-Syntaxin 1A + Syntaxin 1B2 antibody (ab39986) at 0.003 µg/ml (for 1 hour) + Mouse Brain lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 33 kDa
Observed band size : 35 kDa (why is the actual band size different from the predicted?)
0
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