Anti-Syntaxin 1a (phospho S14) antibody (ab63571)
Key features and details
- Rabbit polyclonal to Syntaxin 1a (phospho S14)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Syntaxin 1a (phospho S14) antibody
See all Syntaxin 1a primary antibodies -
Description
Rabbit polyclonal to Syntaxin 1a (phospho S14) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human Syntaxin 1a (phospho S14).
Database link: Q16623 -
General notes
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63571 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis. May mediate Ca(2+)-regulation of exocytosis acrosomal reaction in sperm. -
Tissue specificity
Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain. -
Involvement in disease
Note=STX1A is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. -
Sequence similarities
Belongs to the syntaxin family.
Contains 1 t-SNARE coiled-coil homology domain. -
Cellular localization
Secreted and Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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Database links
- Entrez Gene: 20907 Mouse
- SwissProt: O35526 Mouse
- Unigene: 6225 Mouse
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Alternative names
- HPC 1 antibody
- Neuron specific antigen HPC1 antibody
- Neuron-specific antigen HPC-1 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab63571 has been referenced in 5 publications.
- Barak-Broner N et al. CK2 Phosphorylation Is Required for Regulation of Syntaxin 1A Activity in Ca2+-Triggered Release in Neuroendocrine Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34948351
- Marcelli S et al. The selective disruption of presynaptic JNK2/STX1a interaction reduces NMDA receptor-dependent glutamate release. Sci Rep 9:7146 (2019). PubMed: 31073146
- Cartier E et al. Rare autism-associated variants implicate syntaxin 1 (STX1 R26Q) phosphorylation and the dopamine transporter (hDAT R51W) in dopamine neurotransmission and behaviors. EBioMedicine 2:135-146 (2015). WB . PubMed: 25774383
- Gil C et al. Protein kinase CK2 associates to lipid rafts and its pharmacological inhibition enhances neurotransmitter release. FEBS Lett 585:414-20 (2011). WB ; Rat . PubMed: 21187092
- Rickman C & Duncan RR Munc18/Syntaxin interaction kinetics control secretory vesicle dynamics. J Biol Chem 285:3965-72 (2010). WB, ICC/IF ; Rat . PubMed: 19748891