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Goat polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (Cy3 ®), pre-adsorbed (ab6969)

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Overview

  • Product nameGoat polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (Cy3 ®), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesSyrian Hamster
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA more details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rabbit, Rat, Sheep more details
  • Immunogen

    Full length native Golden Syrian Hamster IgG (purified).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Hamster IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
  • Conjugation notesCy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.1 moles Cy3 per mole of Goat IgG (acceptable range 6-20)
  • Clonality Polyclonal
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF
IHC-P
IHC-Fr
ICC
Flow Cyt
ELISA
  • Application notesFlow Cyt: Use at an assay dependant dilution.
    ICC: Use at an assay dependant dilution.
    IF: Use at an assay dependant dilution.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for Goat polyclonal Secondary Antibody to Syrian Hamster IgG - H&L (Cy3 ®), pre-adsorbed (ab6969)

    ab6969 has not yet been referenced specifically in any publications.

    Publishing research using ab6969 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab6969 Submit a Question for ab6969

    Displaying 1 - 2 of 2 results for Abreviews and Q&A

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    What kind of hamster is this secondary made to? I want armenian, not gold or syrian.

    I have received an answer about ab33483 and it was raised in Armenian hamsters. I apologize for the delay in getting the answer back for this particular antibody. I hope that this information helps and please do not hesitate to contact us in the futur...

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    I am looking for a Cy3-conjugated Armenian Hamster secondary antibody. You have a Hamster IgG antibody (ab6969). I would like to know if it could work for immunofluorescence as well as an armenian hamster antibody.

    The antibody is made against Golden Syrian Hamster IgG. It has very minimium cross reactivity to Armenian Hamster IgG. It can be used in Immunofluorescence to detect GS hamster IgG though.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"