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Anti-T Cell antibody [KEN-5] (ab34043)

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Overview

Product name

Anti-T Cell antibody [KEN-5]

Description

Mouse monoclonal [KEN-5] to T Cell

Specificity

Recognises >90% thymocytes and 40-45% mesenteric lymph node cells. There is little evidence of B cell activity. Originally thought to recognise rabbit CD5 (Kotani et al., 1993) but does not bind to rabbit CD5 transfectants. Known rabbit CD5 antibodies also show binding to most B lymphocytes, which are not labelled by this clone.

Tested applications

Flow Cyt, IHC-Fr, IPmore details

Cross reactivity

Reacts with

Rabbit

Immunogen

Rabbit thymocytes.

Positive control

Rabbit thymocytes.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: 5-10% Foetal calf serum, 0.1M Tris HCl. pH 7.4

Purity

Tissue culture supernatant

Primary antibody notes

This antibody immunoprecipitates a 67kDa protein from thymocytes.

Clonality

Monoclonal

Clone number

KEN-5

Myeloma

PAI

Isotype

IgG1

Research areas

Immunology >> Adaptive Immunity >> T Cells >> Non-CD

  • Flow Cytometry / FACS - T Cell antibody [KEN-5] (ab34043)Flow Cytometry / FACS - T Cell antibody [KEN-5] (ab34043) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab34043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: Neat. Use 10µl to label 106 cells in 100µl.
IHC-Fr: Neat.
IP: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

A type of white blood cell derived from the thymus. T cells are of key importance to the immune system and are at the core of adaptive immunity, the system that tailors the body's immune response to specific pathogens. T cells do not produce antibodies but do produce a number of substances that regulate the immune response. There are two main subtypes: the CD4+ helper T cells and the CD8+ cytotoxic or suppressor T cells.

Cellular localization

Cell membrane

Alternative names

  • T lymphocytes antibody

Anti-T Cell antibody [KEN-5] images:

  Flow Cytometry / FACS - T Cell antibody [KEN-5] (ab34043)

Flow Cytometry / FACS - T Cell antibody [KEN-5] (ab34043)

Staining of rabbit peripheral blood lymphocytes with mouse anti-rabbit T cell antibody (ab34043).

References for Anti-T Cell antibody [KEN-5] (ab34043)

ab34043 has not yet been referenced specifically in any publications.

Publishing research using ab34043? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"