Anti-T-bet / Tbx21 antibody [39D, 3-9D] (ab91103)
Key features and details
- Mouse monoclonal [39D, 3-9D] to T-bet / Tbx21
- Suitable for: WB, ICC, ChIP
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-T-bet / Tbx21 antibody [39D, 3-9D]
See all T-bet / Tbx21 primary antibodies -
Description
Mouse monoclonal [39D, 3-9D] to T-bet / Tbx21 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC, ChIPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to T-bet/ Tbx21. Recombinant protein purified from E.coli
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Positive control
- WB: Th1-polarized mouse CD4+ T cells, NK-92 cell lysate ICC: NK-92 cells ChIP: NK-92 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
39D, 3-9D -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab91103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 58 kDa.
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ICC |
Use a concentration of 5 µg/ml.
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ChIP |
Use 2.5µg for 106 cells.
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Notes |
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WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 58 kDa. |
ICC
Use a concentration of 5 µg/ml. |
ChIP
Use 2.5µg for 106 cells. |
Target
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Function
Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs. -
Tissue specificity
T-cell specific. -
Involvement in disease
Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities. -
Sequence similarities
Contains 1 T-box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 30009 Human
- Entrez Gene: 57765 Mouse
- Omim: 604895 Human
- SwissProt: Q9UL17 Human
- SwissProt: Q9JKD8 Mouse
- Unigene: 272409 Human
- Unigene: 94519 Mouse
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Alternative names
- T bet antibody
- T box 21 antibody
- T box expressed in T cells antibody
see all
Images
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All lanes : Anti-T-bet / Tbx21 antibody [39D, 3-9D] (ab91103) at 2 µg/ml
Lane 1 : Lysates from control cells
Lane 2 : Lysates from PMA and Ionomycin-re-activated cells
Secondary
All lanes : HRP anti-mouse IgG
Predicted band size: 58 kDa
CD4+ T cells were sorted from mouse spleen, activated with anti-mouse CD3 and anti-mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. -
All lanes : Anti-T-bet / Tbx21 antibody [39D, 3-9D] (ab91103) at 2 µg/ml
Lane 1 : NK-92 cell lysates
Lane 2 : K-562 cell lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant, HRP at 1/4000 dilution
Predicted band size: 58 kDa -
Immunocytochemistry analysis of T-bet/ Tbx21 was performed using 70% confluent log phase NK-92 cells. The cells were fixed with 4% paraformaldehyde for 10 Minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ab91103 at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 at a dilution of 1/2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
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Chromatin Immunoprecipitation (ChIP) assay of endogenous T-bet protein using Anti-T-bet Antibody: ChIP was performed using ab91103 at 5 µg on sheared chromatin from NK-92 cells using the MAGnify ChIP System kit. Normal Mouse IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to IFNG promoter, IKZF3 and CD19 transcriptional start site and SAT2 satellite repeats. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
Datasheets and documents
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Datasheet download
References (2)
ab91103 has been referenced in 2 publications.
- Spurlock CF et al. Profiles of Long Noncoding RNAs in Human Naive and Memory T Cells. J Immunol 199:547-558 (2017). PubMed: 28600289
- Shao YY et al. The Anti-Allergic Rhinitis Effect of Traditional Chinese Medicine of Shenqi by Regulating Mast Cell Degranulation and Th1/Th2 Cytokine Balance. Molecules 22:N/A (2017). PubMed: 28327534