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Products:Immunology >> Adaptive Immunity >> B Cells >> Non-CD
Overview
Product name
Anti-T-bet / Tbx21 antibody
See all T-bet / Tbx21 products (5) ...
Description
Rabbit polyclonal to T-bet / Tbx21
Tested applications
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human T-bet / Tbx21.
(Peptide available as ab69655.)
Positive control
This antibody gave a positive signal in the following lysates: Jurkat Whole Cell, Jurkat Nuclear, Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM), E14Tg2a Whole Cell
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Hematopoietic Progenitors >> Surface Molecules
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Immunology >> Adaptive Immunity >> T Cells >> Non-CD
Immunology >> Adaptive Immunity >> B Cells >> Non-CD
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Western blot - T-bet / Tbx21 antibody (ab53174)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab53174 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 53 kDa (predicted molecular weight: 58 kDa).
Target
Function
Transcription factor that controls the expression of the TH1 cytokine, interferon-gamma. Initiates TH1 lineage development from naive TH precursor cells both by activating TH1 genetic programs and by repressing the opposing TH2 programs.
Tissue specificity
T-cell specific.
Involvement in disease
Genetic variations in TBX21 are associated with susceptibility to asthma with nasal polyps and aspirin intolerance (ANPAI) [MIM:208550]. A condition consisting of asthma, aspirin sensitivity and nasal polyposis. Nasal polyposis is due to chronic inflammation of the paranasal sinus mucosa, leading to protrusion of edematous polyps into the nasal cavities.
Sequence similarities
Contains 1 T-box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ9UL17
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- T bet antibody
- T box 21 antibody
- T box expressed in T cells antibody
see all
Database links
- Entrez Gene: 30009 Human
- Entrez Gene: 57765 Mouse
- Omim: 604895 Human
- SwissProt: Q9UL17 Human
- SwissProt: Q9JKD8 Mouse
- Unigene: 272409 Human
- Unigene: 94519 Mouse
Anti-T-bet / Tbx21 antibody images:
Western blot - T-bet / Tbx21 antibody (ab53174)
All lanes : Anti-T-bet / Tbx21 antibody (ab53174) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 3 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 58 kDa
Observed band size : 53 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
References for Anti-T-bet / Tbx21 antibody (ab53174)
ab53174 has not yet been referenced specifically in any publications.
Publishing research using ab53174? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"