Biotin Anti-T7 tag® antibody (ab81661)
Key features and details
- Biotin Rabbit polyclonal to T7 tag®
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-T7 tag® antibody
See all T7 tag® primary antibodies -
Description
Biotin Rabbit polyclonal to T7 tag® -
Host species
Rabbit -
Conjugation
Biotin -
Specificity
ab81661 recognizes the T7-tag® (MASMTGGQQMG). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.013% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3025% Tris -
Concentration information loading...
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Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Predicted molecular weight: 1 kDa.
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Notes |
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WB
1/5000. Predicted molecular weight: 1 kDa. |
Target
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Relevance
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
Alternative names
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
Images
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All lanes : Biotin Anti-T7 tag® antibody (ab81661) at 0.5 µg/ml
Lane 1 : 60 kDa T7-tagged protein at 0.3 µg
Lane 2 : 60 kDa T7-tagged protein at 0.01 µg
Lane 3 : 60 kDa T7-tagged protein at 0.03 µg
Lane 4 : 60 kDa T7-tagged protein at 0.001 µg
Lane 5 : 60 kDa T7-tagged protein at 0.003 µg
Predicted band size: 1 kDaBlot incubated with primary overnight followed by three washes. Blot stained with 40 ng/mL Streptavidin HRP conjugate and imaged using West Pico chemilumiescent substrate.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab81661 has not yet been referenced specifically in any publications.