DyLight® 550 Anti-T7 tag® antibody (ab117595)
Key features and details
- DyLight® 550 Rabbit polyclonal to T7 tag®
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Species independent
- Conjugation: DyLight® 550. Ex: 562nm, Em: 576nm
- Isotype: IgG
Overview
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Product name
DyLight® 550 Anti-T7 tag® antibody
See all T7 tag® primary antibodies -
Description
DyLight® 550 Rabbit polyclonal to T7 tag® -
Host species
Rabbit -
Conjugation
DyLight® 550. Ex: 562nm, Em: 576nm -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. The product can be stored for up to 12 months. -
Storage buffer
pH: 8.20
Constituent: 99% Tris buffered saline -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab117595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/500 - 1/5000.
|
|
Flow Cyt |
Use at an assay dependent concentration.
|
Notes |
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ICC/IF
1/500 - 1/5000. |
Flow Cyt
Use at an assay dependent concentration. |
Target
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Relevance
The T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins. -
Alternative names
- 10 antibody
- Major capsid protein 10A antibody
- Major capsid protein antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab117595 has been referenced in 1 publication.
- Xu H et al. Construction of a T7 phage display nanobody library for bio-panning and identification of chicken dendritic cell-specific binding nanobodies. Sci Rep 12:12122 (2022). PubMed: 35840654