Anti-T7 tag® antibody (ab9115)
- Product nameAnti-T7 tag® antibodySee all T7 tag® primary antibodies ...
- DescriptionRabbit polyclonal to T7 tag®
- SpecificityELISA: The anti T7 diluted 1:176,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
- Tested applicationsICC/IF, ELISA, WB more details
MASMTGGQQMG (T7 epitope) conjugated to KLH.
- General notesT7-TAG® is a trademark of EMD Biosciences, Inc.
- Storage instructionsStore at +4°C.
- Storage bufferPBS, pH 7.2, sodium azide, 0.1%
- Concentration information loading...
- PurityIgG fraction
- Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
- Clonality Polyclonal
- Light chain typeunknown
- Research Areas
Our Abpromise guarantee covers the use of ab9115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||ICC/IF: Use at an assay dependent concentration.|
|ELISA||ELISA: Use at an assay dependent concentration.|
|WB||WB: Use at an assay dependent concentration.|
- RelevanceThe T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
- 10 antibodyMajor capsid protein 10A antibodyMASMTGGQQMG epitope tag antibody
- MASMTGGQQMG tag antibodyMinor capsid protein 10B antibodyT7 epitope tag antibody
References for Anti-T7 tag® antibody (ab9115)
This product has been referenced in:
- Kubota T et al. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J Biol Chem 283:25660-70 (2008). WB . Read more (PubMed: 18635538) »