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Overview

  • Product nameAnti-T7 tag® antibodySee all T7 tag® primary antibodies ...
  • Description
    Rabbit polyclonal to T7 tag®
  • SpecificityELISA: The anti T7 diluted 1:176,000 gave an O.D.=1.0 in a 15 minute reaction against peptide conjugated with a different carrier than used for anti peptide purification.
  • Tested applicationsICC/IF, ELISA, WB more details
  • Immunogen

    MASMTGGQQMG (T7 epitope) conjugated to KLH.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notesT7-TAG® is a trademark of EMD Biosciences, Inc.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPBS, pH 7.2, sodium azide, 0.1%
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesAntibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support.
  • Clonality Polyclonal
  • Isotypeunknown
  • Light chain typeunknown
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab9115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use at an assay dependent concentration.
ELISA ELISA: Use at an assay dependent concentration.
WB WB: Use at an assay dependent concentration.

Target

  • RelevanceThe T7 tag is an 11 amino acid peptide encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. The T7 tag serves as a tag in many expression vectors including the pET system that is based on the very efficient T7 RNA polymerase expression system. Monoclonal antibodies specific for T7 tag are an important tool for studying expression of recombinant T7-tagged proteins.
  • Alternative names
      10 antibodyMajor capsid protein 10A antibodyMASMTGGQQMG epitope tag antibody
      MASMTGGQQMG tag antibodyMinor capsid protein 10B antibodyT7 epitope tag antibody
    see all

References for Anti-T7 tag® antibody (ab9115)

This product has been referenced in:
  • Kubota T  et al. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J Biol Chem 283:25660-70 (2008). WB . Read more (PubMed: 18635538) »

See 1 Publication for this product

Publishing research using ab9115 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab9115 Submit a Question for ab9115

Displaying 1 - 4 of 4 results for Abreviews and Q&A

10
Votes: Highest First

Dear Mr./Ms,

i'm looking for anti-T7 and anti-HIS antibodies for WB and i need some informations.
i'm thinking to order anti-T7 tag antidoby (ab9115) and Anti-6X His tag® antibody [HIS.H8] (ab18184)

technical informations: <...

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Thank you for contacting us.

Ab9115 and ab18184 are provided at 1mg/ml. For both antibodies I would recommend an initial dilution 1/1000 and try serial dilutions in order to optimise it to your experiment.

You can also have a look at t...

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Immunocytochemistry/ Immunofluorescence abreview for Anti-T7 tag® antibody

 Below Average
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela)
Specification Hela
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
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Username

Verified customer

Submitted Jan 27 2006

Follow up to complaint: Thanks for writing, please let me answer all your questions: 1) we used an N-terminal tag, which also allows recognition by the monoclonal T7 antibody. 2) high copy number (see control blot) 3) 293T cells 4) between 0.2 an...

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Thank you very much for those details, it is very useful. I am sorry you are not satisfied with ab9115. In this instance, without admission of responsibility or liability, I am prepared to offer you a credit note or refund for the value of the antibody...

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is it possible to applicate this ,Rabbit polyclonal to T7 epitope tag, to immunocytochemistry? Or do you have anyother T7 tag antibody to applicate to immunocytochemistry?

This antibody has not yet been tested in ICC. We have two further antibodies to T7 epitope tag (ab9112 an ab9138), but neither of these have been tested in ICC !

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"