Anti-TACD2 antibody [162-46.2] (ab79976)

Overview

• Product nameAnti-TACD2 antibody [162-46.2]See all TACD2 primary antibodies ...
• Description
  Mouse monoclonal [162-46.2] to TACD2
• Specificityab79976 defines TACD2 that is expressed on trophoblasts and choriocarcinoma cells but not on blood cells or other tumor cells.
• Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Human choriocarcinoma cell line BeWo

• Positive control10 week placental tissue.
• General notesIsotype: IgG1 (Igh-4a allotype)

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone number162-46.2
• IsotypeIgG1
• Research Areas
  • Cancer
  • Signal transduction
  • Other

  • Cancer
  • Tumor biomarkers
  • Other

Applications

Target

• FunctionMay function as a growth factor receptor.
• Tissue specificityPlacenta, pancreatic carcinoma cell lines.
• Involvement in diseaseDefects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.
• Sequence similaritiesBelongs to the EPCAM family.
  Contains 1 thyroglobulin type-1 domain.
• Post-translational
  modificationsThe N-terminus is blocked.
• Cellular localizationMembrane.

Target information above from: UniProt accession P09758 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4070 Human
  • Omim: 137290 Human
  • SwissProt: P09758 Human
  • Unigene: 23582 Human

• Alternative names
  Cell surface glycoprotein Trop 2 antibodyCell surface glycoprotein Trop-2 antibodyCell surface glycoprotein Trop2 antibody

  Epithelial glycoprotein 1 antibodyGA733 1 antibodyGA7331 antibodyM1S 1 antibodyM1S1 antibodyMembrane component chromosome 1 surface marker 1 antibodyPancreatic carcinoma marker protein GA733 1 antibodyPancreatic carcinoma marker protein GA733-1 antibodyPancreatic carcinoma marker protein GA7331 antibodyTACD 2 antibodyTACD2_HUMAN antibodyTACSTD 2 antibodyTACSTD2 antibodyTrop 2 antibodyTrop2 antibodyTumor associated calcium signal transducer 2 precursor antibodyTumor-associated calcium signal transducer 2 antibody

  see all

Anti-TACD2 antibody [162-46.2] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TACD2 antibody (ab79976)
  ab79976 staining TACD2 in human 10 week placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Anti-mouse peroxidase IgG used as secondary antibody. Note staining of cytotrophoblast (CT) and syncytiotrophoblast (ST).
• 

  Immunocytochemistry/ Immunofluorescence-TACD2 antibody [162-46.2](ab79976)
  ICC/IF image of ab79976 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Flow Cytometry - Anti-TACD2 antibody [162-46.2] (ab79976)
  Overlay histogram showing MCF7 cells stained with ab79976 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed MCF7 cells used under the same conditions.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.