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Anti-TADA3L antibody [TADAD25A] (ab51024)

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Overview

Product name

Anti-TADA3L antibody [TADAD25A]
See all TADA3L products (4) ...

Description

Mouse monoclonal [TADAD25A] to TADA3L

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment (N-terminal)(Human).

Positive control

HeLa whole cell lysate

Properties

Form

Liquid

Storage instructions

Store at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.

Clonality

Monoclonal

Clone number

TADAD25A

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Other factors

  • Western blot - TADA3L antibody [TADAD25A] (ab51024)Western blot - TADA3L antibody [TADAD25A] (ab51024) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab51024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

TADA3L functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. TADA3L is also known as a coactivator for p53/TP53-dependent transcriptional activation. The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3.

Cellular localization

Nuclear

Database links

Anti-TADA3L antibody [TADAD25A] images:

  Western blot - TADA3L antibody [TADAD25A] (ab51024)

Western blot - TADA3L antibody [TADAD25A] (ab51024)

Anti-TADA3L antibody [TADAD25A] (ab51024) at 1/50 dilution + HeLa whole cell lysate at 50 µg

Secondary
Mouse IgG antibody at 1/2500 dilution
developed using the ECL technique

Predicted band size : 49 kDa
Observed band size : 49 kDa
Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TADA3L antibody [TADAD25A] (ab51024)

ab51024 has not yet been referenced specifically in any publications.

Publishing research using ab51024? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"