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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-TADA3L antibody
See all TADA3L products (4) ...
Description
Rabbit polyclonal to TADA3L
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (AAVADKKKGL MGPLTELDTK DVDALLKKSE AQHEQPEDGC PFGALTQRLL) of Human TADA3L (NP_006345).
AAVADKKKGL MGPLTELDTK DVDALLKKSE AQHEQPEDGC PFGALTQRLL
Positive control
293T cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - TADA3L antibody (ab83270)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83270 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
- 1 Image
WB
Application notes
ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/62500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
TADA3L functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. TADA3L is also known as a coactivator for p53/TP53-dependent transcriptional activation. The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3.
Cellular localization
Nuclear
Alternative names
- ADA3 antibody
- ADA3 homolog antibody
- ADA3-like protein antibody
see all
Database links
- Entrez Gene: 510184 Cow
- Entrez Gene: 10474 Human
- Entrez Gene: 101206 Mouse
- Entrez Gene: 362414 Rat
- Entrez Gene: 334785 Zebrafish
- Omim: 602945 Human
- SwissProt: Q5EAE2 Cow
- SwissProt: O75528 Human
see all
Anti-TADA3L antibody images:
Western blot - TADA3L antibody (ab83270)
Anti-TADA3L antibody (ab83270) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 49 kDa
Observed band size : 55 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
References for Anti-TADA3L antibody (ab83270)
ab83270 has not yet been referenced specifically in any publications.
Publishing research using ab83270? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"