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Anti-TAF1 48 antibody (ab72065)

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Overview

Product name

Anti-TAF1 48 antibody
See all TAF1 48 products (4) ...

Description

Rabbit polyclonal to TAF1 48

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Rat

Immunogen

Synthetic peptide derived from an internal sequence of human TAF1 48.

Positive control

Extracts from HeLa cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol I Transcription

  • Western blot - TAF1 48 antibody (ab72065)Western blot - TAF1 48 antibody (ab72065) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab72065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

  • ELISA

     ELISA: 1/10000

    ELISA: 1/10000

Target

Relevance

Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA binding protein (TBP) and three TBP associated factors (TAFs) specific for RNA polymerase I. This complex, also known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the smallest SL1-specific TAF. Two transcripts encoding different isoforms have been identified.

Cellular localization

Nuclear

Alternative names

  • RAFI48 antibody
  • TAFI48 antibody
  • TATA box binding protein associated factor 1A antibody
  • TATA box binding protein associated factor RNA polymerase I subunit A antibody
  • TBP associated factor RNA polymerase I 48 kDa antibody
  • Transcription factor SL1 antibody
see all

Database links

Anti-TAF1 48 antibody images:

  Western blot - TAF1 48 antibody (ab72065)

Western blot - TAF1 48 antibody (ab72065)

All lanes : Anti-TAF1 48 antibody (ab72065) at 1/500 dilution

Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HeLa cells with immunising peptide at 5 µg

Lysates/proteins at 5 µg per lane.


Predicted band size : 53 kDa
Observed band size : 53 kDa
Additional bands at : 29 kDa. We are unsure as to the identity of these extra bands.

References for Anti-TAF1 48 antibody (ab72065)

ab72065 has not yet been referenced specifically in any publications.

Publishing research using ab72065? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"