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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Overview
Product name
Anti-TAF12 antibody
See all TAF12 products (2) ...
Description
Mouse polyclonal to TAF12
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length protein, corresponding to amino acids 1-162 of Human TAF12
Positive control
Transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
-
Western blot - TAF12 antibody (ab66990)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 16 kDa (predicted molecular weight: 18 kDa).
Target
Relevance
TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP free TAFII complex (TFTC). TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I.
Cellular localization
Nuclear
Alternative names
- TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa antibody
- TAF15 antibody
- TAF2J antibody
see all
Database links
- Entrez Gene: 6883 Human
- Omim: 600773 Human
- SwissProt: Q16514 Human
Anti-TAF12 antibody images:
Western blot - TAF12 antibody (ab66990)
All lanes : Anti-TAF12 antibody (ab66990) at 1/500 dilution
Lane 1 : TAF12 transfected 293T cell lysate
Lane 2 : non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size : 18 kDa
Observed band size : 16 kDa (why is the actual band size different from the predicted?)
References for Anti-TAF12 antibody (ab66990)
ab66990 has not yet been referenced specifically in any publications.
Publishing research using ab66990? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"