![Western blot - TAF15 antibody [TAF15B11A6] (ab50767) image](#taf15.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Overview
Product name
Anti-TAF15 antibody [TAF15B11A6]
See all TAF15 products (7) ...
Description
Mouse monoclonal [TAF15B11A6] to TAF15
Tested applications
WB, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment of TAF15
Properties
Form
Liquid
Storage instructions
Store at 4°C for up to one year. Upon use, aliquot and store at -20°C or -80°C.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
Clonality
Monoclonal
Clone number
TAF15B11A6
Isotype
IgG1
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Western blot - TAF15 antibody [TAF15B11A6] (ab50767)
![Western blot - TAF15 antibody [TAF15B11A6] (ab50767) image](/ps/datasheet/Images/50/ab50767/taf15.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 62 kDa.
Dot: Use at an assay dependent dilution.
Target
Function
RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
Tissue specificity
Ubiquitous. Observed in all fetal and adult tissues.
Involvement in disease
Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
Sequence similarities
Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
Post-translational
modifications
Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
Target information above from: UniProt accessionQ92804
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 68 kDa TATA-binding protein-associated factor antibody
- Npl3 antibody
- RBP56 antibody
see all
Database links
- Entrez Gene: 8148 Human
- Omim: 601574 Human
- SwissProt: Q92804 Human
- Unigene: 402752 Human
Anti-TAF15 antibody [TAF15B11A6] images:
Western blot - TAF15 antibody [TAF15B11A6] (ab50767)
Anti-TAF15 antibody [TAF15B11A6] (ab50767) + immunized recombinant protein fragment
Predicted band size : 62 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
References for Anti-TAF15 antibody [TAF15B11A6] (ab50767)
ab50767 has not yet been referenced specifically in any publications.
Publishing research using ab50767? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"