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Western blot - TAF15 antibody (ab69581)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TAF15 antibody (ab69581)
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Product Name
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TAF15 antibody
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See all TAF15 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TAF15
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Immunogen
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Synthesized peptide derived from an internal region of human TAF15.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/5000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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IHC-P: Human brain tissue
WB: Extracts from HepG2 cells
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TAFs
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
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Relevance
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TAF 15 belongs to the RNA polymerase II (Pol II) transcriptional multiprotein complex, together with the TATA-binding protein (TBP) and other TBP-associated factors (TAF(II)s). It is an RNA and ssDNA binding protein that may play specific roles during transcription initiation at distinct promoters, and can enter the preinitiation complex together with RNA polymerase II (Pol II).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TAF15 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TAF15 antibody images, references, products related to ab69581 and other tools.
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TAF15 antibody images:
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 Western blot - TAF15 antibody (ab69581)
All lanes : TAF15 antibody (ab69581) at 1/500 dilution
Lane 1 : extracts from HepG2 cells, without immunising peptide
Lane 2 : extracts from HepG2 cells, with immunising peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 62 kDa
Observed band size : 62 kDa
Additional bands at : 70 kDa,>117 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TAF15 antibody (ab69581)
Immunohistochemical analysis of TAF15 in paraffin embedded
human brain tissue using 1/50 ab69581. Left image: untreated sample. Right image: sample treated with immunising peptide.
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Search PubMed (MEDLINE) for references to TAF15
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TAF15 antibody - more information
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Customer reviews (feedback) regarding TAF15 antibody |
Customer FAQs regarding TAF15 antibody |
Protocols for TAF15 antibody |
Price and availability of products related to TAF15 antibody |
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TAF15 antibody - related products:
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TAF15 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TAF15 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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