Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Overview
Product name
Anti-TAF15 antibody
See all TAF15 products (7) ...
Description
Rabbit polyclonal to TAF15
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthesized peptide derived from an internal region of human TAF15.
Positive control
IHC-P: Human brain tissue WB: Extracts from HepG2 cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab69581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ELISA
ELISA: 1/5000ELISA: 1/5000
IHC-P: 1/50 - 1/100.
Target
Function
RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
Tissue specificity
Ubiquitous. Observed in all fetal and adult tissues.
Involvement in disease
Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
Sequence similarities
Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
Post-translational
modifications
Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
Target information above from: UniProt accessionQ92804
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 68 kDa TATA-binding protein-associated factor antibody
- Npl3 antibody
- RBP56 antibody
see all
Database links
- Entrez Gene: 8148 Human
- Entrez Gene: 70439 Mouse
- Omim: 601574 Human
- SwissProt: Q92804 Human
- SwissProt: Q8BQ46 Mouse
- Unigene: 402752 Human
Anti-TAF15 antibody images:
Western blot - TAF15 antibody (ab69581)
All lanes : Anti-TAF15 antibody (ab69581) at 1/500 dilution
Lane 1 : extracts from HepG2 cells, without immunising peptide
Lane 2 : extracts from HepG2 cells, with immunising peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 62 kDa
Observed band size : 62 kDa
Additional bands at : 70 kDa,>117 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TAF15 antibody (ab69581)
Immunohistochemical analysis of TAF15 in paraffin embedded human brain tissue using 1/50 ab69581. Left image: untreated sample. Right image: sample treated with immunising peptide.
References for Anti-TAF15 antibody (ab69581)
ab69581 has not yet been referenced specifically in any publications.
Publishing research using ab69581? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"