Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
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All lanes : Anti-TAF15 antibody (ab70389) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells
at 200 µg
Lane 2 : Whole cell lysate from 293T cells immunoprecipitated with rabbit IgG control.
Lane 3 : Whole cell lysate from 293T cells immunoprecipitated with 0.3 µg/ml ab70389.
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa (possible IgG),60 kDa (possible IgG).
10 mg of Whole cell lysate from 293T cells was used for IP.
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