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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Overview
Product name
Anti-TAF15 antibody
See all TAF15 products (7) ...
Description
Rabbit polyclonal to TAF15
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 175 and 225 of Human TAF15 (SwissProt Q92804)
RGYGGS QGGGRGRGGY DKDGRGPMTG SSGGDRGGFK NFGGHRDYGP RTDAD
Positive control
Whole cell lysate from 293T cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: Tris buffered saline, pH 7 to 8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - TAF15 antibody (ab70389)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
IP
Application notes
IP: Use at 1 - 4 µg/mg of lysate.
WB: 1/5000 - 1/20000. Detects a band of approximately 70 kDa (predicted molecular weight: 62 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
Tissue specificity
Ubiquitous. Observed in all fetal and adult tissues.
Involvement in disease
Note=A chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with NR4A3.
Sequence similarities
Belongs to the RRM TET family.
Contains 1 RanBP2-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
Post-translational
modifications
Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. Cytoplasm. Shuttles from the nucleus to the cytoplasm.
Target information above from: UniProt accessionQ92804
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 68 kDa TATA-binding protein-associated factor antibody
- AF15 RNA polymerase II, TATA box binding protein (TBP) associated factor, 68 kD antibody
- Npl3 antibody
see all
Database links
- Entrez Gene: 8148 Human
- Omim: 601574 Human
- SwissProt: Q92804 Human
- Unigene: 402752 Human
Anti-TAF15 antibody images:
Western blot - TAF15 antibody (ab70389)
All lanes : Anti-TAF15 antibody (ab70389) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells
at 200 µg
Lane 2 : Whole cell lysate from 293T cells immunoprecipitated with rabbit IgG control.
Lane 3 : Whole cell lysate from 293T cells immunoprecipitated with 0.3 µg/ml ab70389.
Predicted band size : 62 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
Additional bands at : 30 kDa (possible IgG),60 kDa (possible IgG).
10 mg of Whole cell lysate from 293T cells was used for IP.
References for Anti-TAF15 antibody (ab70389)
ab70389 has not yet been referenced specifically in any publications.
Publishing research using ab70389? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"