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Western blot - TAF15 antibody (ab70390)
(enlarge)
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Product Name
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TAF15 antibody
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See all TAF15 antibodies (5)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TAF15
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Immunogen
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Synthetic peptide corresponding to region between residue 550 and the C-terminus (residue 592) of Human TAF15 (Swiss-Prot entry Q92804)
BLAST 'G GDRSGGGY GG DRGGGYG GDR GGYGGK MGGR NDYRN ' with  or 
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Reacts with
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Hu
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 1 - 4 µg/mg of lysate.
WB: 1/10000 - 1/30000. Detects a band of approximately 66 kDa (predicted molecular weight: 62 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysate from 293T cells.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TAFs
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
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Relevance
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TAF 15 belongs to the RNA polymerase II (Pol II) transcriptional multiprotein complex, together with the TATA-binding protein (TBP) and other TBP-associated factors (TAF(II)s). It is an RNA and ssDNA binding protein that may play specific roles during transcription initiation at distinct promoters, and can enter the preinitiation complex together with RNA polymerase II (Pol II).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: Tris, pH 7 to 8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TAF15 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TAF15 antibody images, references, products related to ab70390 and other tools.
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TAF15 antibody images:
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 Western blot - TAF15 antibody (ab70390)
TAF15 antibody (ab70390) at 1/20000 dilution + Whole cell lysate from 293T cells at 200 µg
developed using the ECL technique
Predicted band size : 62 kDa
Observed band size : 66 kDa (why is the actual band size different from the predicted?)
Additional bands at : 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 5 seconds
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Search PubMed (MEDLINE) for references to TAF15
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TAF15 antibody - more information
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Customer reviews (feedback) regarding TAF15 antibody |
Customer FAQs regarding TAF15 antibody |
Protocols for TAF15 antibody |
Price and availability of products related to TAF15 antibody |
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TAF15 antibody - related products:
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TAF15 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TAF15 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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