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Anti-TAF1B antibody (ab66395)

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Overview

Product name

Anti-TAF1B antibody
See all TAF1B products (3) ...

Description

Rabbit polyclonal to TAF1B

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Mouse

Predicted to work with

Rat

Immunogen

A region within synthetic peptide: YQFILNIFSF LLRIKTSALH EEVSLLEKKL FEKKYNESKK SSGSKKGRRH, corresponding to C terminal amino acids 537-586 of Mouse TAF1B

YQFILNIFSF LLRIKTSALH EEVSLLEKKL FEKKYNESKK SSGSKKGRRH

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

SP2/0 cell lysate

Properties

Form

Lyophilised:
Add 100 µl of distilled water.

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Preinitiation complex
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TAFs
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol I Transcription

  • Western blot - TAF1B antibody (ab66395)Western blot - TAF1B antibody (ab66395) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab66395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
ELISA titre using peptide based assay: 1:312500

WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 68 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

TAF1B belongs to the family of TATA box binding protein (Tbp)-associated factors. Promoter selectivity for all three classes of eukaryotic RNA polymerases is brought about by multimeric protein complexes containing TATA box binding protein (TBP) and specific TBP-associated factors (TAFs).

Cellular localization

Nuclear

Alternative names

  • MGC:9349 antibody
  • RAFI63 antibody
  • TAF1B TATA box binding protein (TBP) associated factor, RNA polymerase I, B, 63kDa antibody
  • TAFI63 antibody
  • TATA box binding protein associated factor 1B antibody
  • RAF1B antibody
  • SL1 antibody
  • TAB1 B antibody
  • TATA box binding protein associated factor RNA polymerase I subunit B antibody
see all

Database links

Anti-TAF1B antibody images:

  Western blot - TAF1B antibody (ab66395)

Western blot - TAF1B antibody (ab66395)

Anti-TAF1B antibody (ab66395) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + SP2/0 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 68 kDa
Observed band size : 59 kDa (why is the actual band size different from the predicted?)

References for Anti-TAF1B antibody (ab66395)

ab66395 has not yet been referenced specifically in any publications.

Publishing research using ab66395? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"