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Overview

  • Product nameTAF1C protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 247-742 (496 amino acids) of Human TAF1C Isoform 2 with N terminal His tag; Predicted MWt 55 kDa (UniProt Q15572-2).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 163 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesTGISLSPHLPGELAICSRSGAVCLWSPEDGLRQIYRDPET LVFRDSSS WRWADFTAHPRVLTVGDRTGVKMLDTQGPP GCGLLLFRLGAEASCQKG ERVLLTQYLGHSSPKCLPPT LHLVCTQFSLYLVDERLPLVPMLKWNHG LPSPLLLARL LPPPRPSCVQPLLLGGQGGQLQLLHLAEGASVPRLAGP PQSLPSRIDSLPAFPLLEPKIQWRLQERLKAPTIGLAAVV PPLPSAPT PGLVLFQLSAAGDVFYQQLRPQVDSSLRRD AGPPGDTQPDCHAPTASW TSQDTAGCSQWLKALLKVPL APPVWTAPTFTHRQMLGSTELRREEEEG QRLGVLRKAM ARGQLLLQRDLGSLPAAEPPPAPESGLEDKLSERLGEA WAGRGAAWWERQQGRTSEPGRQTRRPKRRTQLSSSFSLSG HVDPSEDT SSPHSPEWPPADALPLPPTTPPSQELTPDA CAQGVPSEQRQMLRDYMA KLPPQRDTPGCATTPP
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92121 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        RNA polymerase I-specific TBP-associated factor 110 kDaSL1Taf1c
        TAF1C_HUMANTAFI110TAFI95TATA box binding protein associated factor 1CTATA box-binding protein-associated factor 1CTATA box-binding protein-associated factor RNA polymerase I subunit CTBP associated factor 1CTBP associated factor RNA polymerase I 95 kDaTBP associated factor, RNA polymerase I, 110-KDTBP-associated factor 1CTranscription initiation factor SL1/TIF-IB subunit C
      see all
  • FunctionComponent of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1/TIF-IB with the rDNA promoter. SL1/TIF-IB is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Formation of SL1/TIF-IB excludes the association of TBP with TFIID subunits. Recruits RNA polymerase I to the rRNA gene promoter via interaction with RRN3.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q15572 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    TAF1C protein (Tagged-His Tag) images

    • SDS-PAGE - TAF1C protein (Tagged-His Tag) (ab92121)
      SDS-PAGE - TAF1C protein (Tagged-His Tag) (ab92121)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for TAF1C protein (Tagged-His Tag) (ab92121)

    ab92121 has not yet been referenced specifically in any publications.

    Publishing research using ab92121 ? Please let us know so that we can cite the reference in this datasheet

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