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Anti-TAF3 antibody (ab21764)

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    Overview

    Product name

    Anti-TAF3 antibody
    See all TAF3 products (3) ...

    Description

    Mouse polyclonal to TAF3

    Tested applications

    WBmore details

    Cross reactivity

    Reacts with

    Fruit fly (Drosophila melanogaster)

    Immunogen

    Fusion protein: MLTVTTPKKN RKQKHDLICE PGQSELLTNP FEKAQEKSQR KALKMYKQTS KNQRDSSINQ IQNMKKLKKK FNRGSFDPNK KHLEKIFKKQ SKLKQNDLQL , corresponding to amino acids 301/400 of Fruit fly (Drosophila melanogaster) TAF3

    MLTVTTPKKN RKQKHDLICE PGQSELLTNP FEKAQEKSQR KALKMYKQTS KNQRDSSINQ IQNMKKLKKK FNRGSFDPNK KHLEKIFKKQ SKLKQNDLQL

    General notes

    Produced from outbred CD1 mice

    Properties

    Form

    Liquid

    Storage instructions

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

    Storage buffer

    Constituents: 50% Glycerol

    Purity

    Whole antiserum

    Primary antibody notes

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

    Clonality

    Polyclonal

    Isotype

    IgG

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab21764 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • WB

       

    Application notes

    WB: 1/1000. Predicted molecular weight: 155 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

    Target

    Relevance

    Taf3 has RNA polymerase activity. TAF3 is required in complex with TBPL2 for the differentiation of myoblasts into myocytes

    Cellular localization

    Nuclear

    Alternative names

    • 140 kDa TATA box-binding protein-associated factor antibody
    • Bip2 protein antibody
    • TAF(II)14 antibody
    • TAF140 antibody
    • TAF3 RNA polymerase II TATA box binding protein (TBP)-associated factor antibody
    • TAFII140 antibody
    • TAFII155 protein antibody
    • TBP-associated factor 3 antibody
    • Transcription initiation factor TFIID 140 kDa subunit antibody
    • Transcription initiation factor TFIID subunit 3 antibody
    see all

    Database links

    References for Anti-TAF3 antibody (ab21764)

    ab21764 has not yet been referenced specifically in any publications.

    Publishing research using ab21764? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"