TAF3 antibody (ab21764)

Product Name 

TAF3 antibody

Product type 

Primary antibodies

Description 

Mouse polyclonal to TAF3

Immunogen 

Fusion protein: MLTVTTPKKN RKQKHDLICE PGQSELLTNP FEKAQEKSQR KALKMYKQTS KNQRDSSINQ IQNMKKLKKK FNRGSFDPNK KHLEKIFKKQ SKLKQNDLQL , corresponding to amino acids 301/400 of Fruit fly (Drosophila melanogaster) TAF3
BLAST 'MLTVTTPKKN RKQKHDLICE PGQSELLTNP FEKAQEKSQR KALKMYKQTS KNQRDSSINQ IQNMKKLKKK FNRGSFDPNK KHLEKIFKKQ SKLKQNDLQL ' with or

Reacts with 
(species key)

Dm

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
WB: 1/1000. Predicted molecular weight: 155 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> RNA polymerase

Relevance 

Taf3 has RNase polymerase activity. TAF3 is required in complex with TBPL2 for the differentiation of myoblasts into myocytes

This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein.

A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein.

Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Database links 

Raised in 

Mouse

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Constituents: 50% Glycerol

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Notes 

Produced from outbred CD1 mice

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