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Western blot - TAF3 antibody (ab85419)
Western blot - TAF3 antibody (ab85419)
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Product Name 

TAF3 antibody

 

See all TAF3 antibodies (2)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to TAF3

Immunogen 

Synthetic peptide corresponding to a region from internal sequence amino acids 540-589 (RDREREKDK NKDKSKEKDK VKEKEKDKET GRETKYPWKE FLKEEEADPY of Human TAF3 (NP_114129)
BLAST 'RDREREKDKN KDKSKEKDKV KEKEKDKETG RETKYPWKEF LKEEEADPYK ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 104 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HepG2 Cell lysate

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> RNA polymerase

Relevance 

Taf3 has RNase polymerase activity. TAF3 is required in complex with TBPL2 for the differentiation of myoblasts into myocytes

 

Alternative names TAF3 antibody (ab85419)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

83860    (Human)

Omim

    

606576    (Human)

SwissProt

    

Q5VWG9    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TAF3 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for TAF3 antibody images, references, products related to ab85419 and other tools.

 

TAF3 antibody images:

Product image  Western blot - TAF3 antibody (ab85419)

Western blot - TAF3 antibody (ab85419)

TAF3 antibody (ab85419) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 Cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 104 kDa
Observed band size : 92 kDa (why is the actual band size different from the predicted?)


Gel concentration: 6-18%

 
 

Search PubMed (MEDLINE) for references to TAF3

 

TAF3 antibody - more information

Customer reviews (feedback) regarding TAF3 antibody

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Price and availability of products related to TAF3 antibody

 

TAF3 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for TAF3 antibody

ab14660

  

HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

 

Compatible Secondaries for TAF3 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

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Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

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Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other TAF3 Antibodies

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TAF3 antibody - other tools:

Contact Abcam with a Technical Enquiry about TAF3 antibody

 

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