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Anti-TAF4B antibody [TAFAD26A] (ab50808)

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Overview

Product name

Anti-TAF4B antibody [TAFAD26A]
See all TAF4B products (2) ...

Description

Mouse monoclonal [TAFAD26A] to TAF4B

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Recombinant Fragment

Predicted to work with

Human

Immunogen

Recombinant human TAF4B

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS (8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate), pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

ab50808 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µn membrane.

Clonality

Monoclonal

Clone number

TAFAD26A

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TAFs
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID

  • Western blot - TAF4B antibody [TAFAD26A] (ab50808)Western blot - TAF4B antibody [TAFAD26A] (ab50808) image (enlarge)

Applications

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Our Abpromise guarantee covers the use of ab50808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

TAF4B is a cell type-specific subunit of TFIID that may function as a gene-selective coactivator in certain cells. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). This protein is preferentially expressed in ovarian granulosa cells and is highly expressed in B cells.

Cellular localization

Nucleus. Cytoplasm. Export into the cytoplasm is mediated by a CRM1-independent nuclear export pathway and not by phosphorylation.

Alternative names

  • TAFII105 antibody
  • TAF2C2 antibody
  • TAF4b RNA polymerase II, TATA box binding protein (TBP) associated factor, 105kDa antibody
  • TAFII105ALPHA antibody
  • TATA box binding protein (TBP) associated factor, RNA polymerase II, C2, 105kD antibody
  • Transcription initiation factor TFIID 105 kD subunit antibody
see all

Database links

Anti-TAF4B antibody [TAFAD26A] images:

  Western blot - TAF4B antibody [TAFAD26A] (ab50808)

Western blot - TAF4B antibody [TAFAD26A] (ab50808)

Anti-TAF4B antibody [TAFAD26A] (ab50808) + Recombinant TAF4B fragment

Predicted band size : 91 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)


This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

References for Anti-TAF4B antibody [TAFAD26A] (ab50808)

ab50808 has not yet been referenced specifically in any publications.

Publishing research using ab50808? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"