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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Overview
Product name
Anti-TAF7L antibody
Description
Rabbit polyclonal to TAF7L
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Human
Immunogen
A region within synthetic peptide: EGDDDDDEDE DDEDYGNEKE EEETDNSEEE LEKELQAKFN EFSLHEADQD, corresponding to internal sequence amino acids 253-302 of Human TAF7L
EGDDDDDEDE DDEDYGNEKE EEETDNSEEE LEKELQAKFN EFSLHEADQD
Positive control
SP2/0 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Preinitiation complex
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TAFs
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
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Western blot - TAF7L antibody (ab49164)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution.
(ELISA titre using peptide based assay: 1:1562500)
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Probably functions as a spermatogensis-specific component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. May play a role in spermatogenesis.
Tissue specificity
Testis-specific.
Sequence similarities
Belongs to the TAF7 family.
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic in spermatogonia and early spermatocytes (preleptotene, leptotene, and zygotene); translocates into the nuclei of pachytene spermatocytes and round spermatids.
Target information above from: UniProt accessionQ5H9L4
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cancer/testis antigen 40 antibody
- CT40 antibody
- FLJ23157 antibody
see all
Database links
- Entrez Gene: 54457 Human
- Entrez Gene: 74469 Mouse
- Omim: 300314 Human
- SwissProt: Q5H9L4 Human
- SwissProt: Q5H9L6 Human
- SwissProt: Q86XI4 Human
- SwissProt: Q9BXU5 Human
- SwissProt: Q9H5R0 Human
see all
Anti-TAF7L antibody images:
Western blot - TAF7L antibody (ab49164)
Anti-TAF7L antibody (ab49164) at 1.25 µg/ml + SP2\0 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 42 kDa
Observed band size : 42 kDa
Gel concentration: 12%
References for Anti-TAF7L antibody (ab49164)
This product has been referenced in:
- D'Alessio JAet al. Core promoter recognition complex changes accompany liver development. Proc Natl Acad Sci U S A 108:3906-11 (2011). WB; Mouse.Read more (PubMed: 21368148) »
See 1 publication for this product
Publishing research using ab49164? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"