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Western blot - TAF9 antibody (ab30963)
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Immunohistochemistry (Paraffin-embedded sections) - TAF9 antibody (ab30963)
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Product Name
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TAF9 antibody
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See all TAF9 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TAF9
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Immunogen
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Synthetic peptide derived from residues 28 - 77 of human TAF9.
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Reacts with
(species key)
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Hu
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Specificity
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ab30963 recognises transcription initiation factor TFIID subunit 9 (TAF9)
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/312500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 0.125 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: fetal small intestine lysate
IHC: human skin
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> RNA polymerase
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
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Relevance
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TAF9 functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TAF9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TAF9 antibody images, references, products related to ab30963 and other tools.
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TAF9 antibody images:
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 Immunohistochemistry (Paraffin-embedded sections) - TAF9 antibody (ab30963)
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Search PubMed (MEDLINE) for references to TAF9
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TAF9 antibody - more information
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Customer reviews (feedback) regarding TAF9 antibody |
Customer FAQs regarding TAF9 antibody |
Protocols for TAF9 antibody |
Price and availability of products related to TAF9 antibody |
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TAF9 antibody - related products:
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TAF9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TAF9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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