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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Overview
Product name
Anti-TAF9 antibody
See all TAF9 products (3) ...
Description
Rabbit polyclonal to TAF9
Specificity
ab30963 recognises transcription initiation factor TFIID subunit 9 (TAF9)
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow, Dog
Immunogen
Synthetic peptide derived from residues 28 - 77 of human TAF9.
.
Positive control
WB: fetal small intestine lysate IHC: human skin
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30963 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.125 µg/mlDetects a band of approximately 20 kDa (predicted molecular weight: 29 kDa).
ELISA
ELISA: 1/312500ELISA: 1/312500
IHC-P: Use a concentration of 4 - 8 µg/ml.
Target
Relevance
TAF9 functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5).
Cellular localization
Nuclear
Alternative names
- AK6 antibody
- CGI 137 antibody
- CINAP antibody
see all
Database links
- Entrez Gene: 6880 Human
- Entrez Gene: 108143 Mouse
- Omim: 600822 Human
- SwissProt: Q16594 Human
- SwissProt: Q9Y3D8 Human
- SwissProt: Q8VI33 Mouse
- Unigene: 248941 Human
- Unigene: 301148 Mouse
Anti-TAF9 antibody images:
Western blot - TAF9 antibody (ab30963)
Anti-TAF9 antibody (ab30963) at 0.125 µg/ml + Fetal small intestine lysate
Secondary
HRP conjugated anti-rabbit IgG
Predicted band size : 29 kDa
Observed band size : 15,20 kDa (why is the actual band size different from the predicted?)
References for Anti-TAF9 antibody (ab30963)
ab30963 has not yet been referenced specifically in any publications.
Publishing research using ab30963? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"