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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
Overview
Product name
Anti-TAF9 antibody
See all TAF9 products (3) ...
Description
Rabbit polyclonal to TAF9
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Syntheric peptide derived from an internal sequence of human TAF9.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - TAF9 antibody (ab65060)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab65060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Predicted molecular weight: 29 kDa.
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Relevance
TAF9 functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5).
Cellular localization
Nuclear
Alternative names
- AK6 antibody
- CGI 137 antibody
- CINAP antibody
see all
Database links
- Entrez Gene: 6880 Human
- Entrez Gene: 108143 Mouse
- Entrez Gene: 373541 Rat
- Omim: 600822 Human
- SwissProt: Q16594 Human
- SwissProt: Q9Y3D8 Human
- SwissProt: Q8VI33 Mouse
- SwissProt: Q5BKE0 Rat
see all
Anti-TAF9 antibody images:
Western blot - TAF9 antibody (ab65060)
All lanes : Anti-TAF9 antibody (ab65060) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells at 5-30ug
Lane 2 : Extracts from HepG2 cells at 5-30ug with immunizing peptide at 5-10ug
Predicted band size : 29 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.
References for Anti-TAF9 antibody (ab65060)
ab65060 has not yet been referenced specifically in any publications.
Publishing research using ab65060? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"