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Western blot - TAF9 antibody (ab65060)
(enlarge)
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Product Name
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TAF9 antibody
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See all TAF9 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TAF9
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Immunogen
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Syntheric peptide derived from an internal sequence of human TAF9.
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Reacts with
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Hu, Ms, Rat
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Tested applications
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ELISA, WB
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Abreviews
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Application notes
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Recommended dilutions
ELISA: 1/20000.
WB: 1/500 - 1/1000. Predicted molecular weight: 29 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> RNA polymerase
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> TFIID
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Relevance
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TAF9 functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription complex SAGA. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3 and SPT8) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3, and TRA1), and chromatin modification through histone acetylation (GCN5).
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TAF9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TAF9 antibody images, references, products related to ab65060 and other tools.
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TAF9 antibody images:
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 Western blot - TAF9 antibody (ab65060)
All lanes : TAF9 antibody (ab65060) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells at 5-30ug
Lane 2 : Extracts from HepG2 cells at 5-30ug with immunizing peptide at 5-10ug
Predicted band size : 29 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to TAF9
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TAF9 antibody - more information
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Customer reviews (feedback) regarding TAF9 antibody |
Customer FAQs regarding TAF9 antibody |
Protocols for TAF9 antibody |
Price and availability of products related to TAF9 antibody |
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TAF9 antibody - related products:
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TAF9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TAF9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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