TAG1 antibody (ab68945)

Product Name 

TAG1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to TAG1

Immunogen 

A synthetic peptide as a part of rat and mouse TAG1 conjugated to an immunogenic carrier protein

Reacts with 
(species key)

Ms, Rat

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/300 - 1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/300 - 1/3000.
WB: 1/300 - 1/3000. Predicted molecular weight: 114 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cell Membrane (GPI-anchored to cell surface).

Research areas 

Cancer >> Invasion/microenvironment >> ECM >> Cell adhesion >> Other
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins

Relevance 

TAG1 (Transient Axonal Glycoprotein 1), also known as contactin 2, is a GPI-anchored protein that is expressed on neurons and glial cells. It comprises 4 fibronectin type III domains and 6 Ig-like C2 domains. It is thought to play a role in the initial growth and guidance of axons, and may also be involved in cell adhesion. TAG1 may also play a role in glial tumorigenesis.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: Whole serum

Form 

Lyophilised
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.

References for TAG1 antibody (ab68945)

  This product has been used in:

Ashique AM et al. The Rfx4 transcription factor modulates Shh signaling by regional control of ciliogenesis. Sci Signal 2:ra70 (2009). PubMed: 19887680

If you publish research using ab68945 please let us know so that we can cite the reference on this datasheet.

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