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All lanes : Anti-TAP1 antibody [53H8] (ab10356) at 5 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 87 kDa
Observed band size : 81 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa (possible non-specific binding).
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