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Western blot - TAP1 antibody [53H8] (ab10356)
![Western blot - TAP1 antibody [53H8] (ab10356)](/ps/datasheet/images/10/ab10356/TAP1-Primary-antibodies-ab10356-2.jpg)
(enlarge)
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Product Name
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TAP1 antibody [53H8]
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See all TAP1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [53H8] to TAP1
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Immunogen
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Unfortunately, this information is considered to be commercially sensitive
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 81 kDa (predicted molecular weight: 87 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane
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Research areas
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Signal Transduction >> Adapters >> Transmembrane
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Relevance
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The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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53H8
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Myeloma
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Sp2/0
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Isotype
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IgG1
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Protein A purified from tissue culture supernatant.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TAP1 antibody [53H8] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TAP1 antibody [53H8] images, references, products related to ab10356 and other tools.
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TAP1 antibody [53H8] images:
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 Western blot - TAP1 antibody [53H8] (ab10356)
All lanes : TAP1 antibody [53H8] (ab10356) at 5 µg/ml
Lane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 87 kDa
Observed band size : 81 kDa (why is the actual band size different from the predicted?)
Additional bands at : 100 kDa (possible non-specific binding).
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References for TAP1 antibody [53H8] (ab10356)
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This product has been used in:
Wang W et al. Imaging and characterizing influenza A virus mRNA transport in living cells. Nucleic Acids Res 36:4913-28 (2008). IP. PubMed: 18653528
If you publish research using ab10356 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to TAP1
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TAP1 antibody [53H8] - more information
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Customer reviews (feedback) regarding TAP1 antibody [53H8] |
Customer FAQs regarding TAP1 antibody [53H8] |
Protocols for TAP1 antibody [53H8] |
Price and availability of products related to TAP1 antibody [53H8] |
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TAP1 antibody [53H8] - related products:
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TAP1 antibody [53H8] - other tools:
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Contact Abcam with a Technical Enquiry about TAP1 antibody [53H8] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - TAP1 antibody [53H8] (ab10356)](/index.html?datasheet=10356#TAP1-Primary-antibodies-ab10356-2.jpg)
