Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Adapters >> Transmembrane
Overview
Product name
Anti-TAP1 antibody
See all TAP1 products (8) ...
Description
Mouse polyclonal to TAP1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fusion protein (tagged): SEHDDERVNF PQRKKKGRGP FRWKYGEGNR RSGRGGSGIR SSRLEEDDGD VAMSDAQDGP RVRYNPYTTR PNRRGDTWHD RDRIHVTVRR DRAPPERGGA , corresponding to amino acids 9-108 of Human TAP1
SEHDDERVNF PQRKKKGRGP FRWKYGEGNR RSGRGGSGIR SSRLEEDDGD VAMSDAQDGP RVRYNPYTTR PNRRGDTWHD RDRIHVTVRR DRAPPERGGA
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: None
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
-
Western blot - TAP1 antibody (ab52781)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 81 kDa).
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.
Involvement in disease
Defects in TAP1 are a cause of bare lymphocyte syndrome type 1 (BLS1) [MIM:604571]; also called HLA class I deficiency. BLS1 is a class I antigen deficiency that is not accompanied by particular pathologic manifestations during the first years of life. Systemic infections have not been described. Chronic bacterial infections, often beginning in the first decade of life, are restricted to the respiratory tract.
Sequence similarities
Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.
Contains 1 ABC transmembrane type-1 domain.
Contains 1 ABC transporter domain.
Domain
The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.
Cellular localization
Endoplasmic reticulum membrane. The transmembrane segments seem to form a pore in the membrane.
Target information above from: UniProt accessionQ03518
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ABC 17 antibody
- ABC transporter MHC 1 antibody
- ABC17 antibody
see all
Database links
- Entrez Gene: 6890 Human
- Omim: 170260 Human
- SwissProt: Q03518 Human
- Unigene: 352018 Human
Anti-TAP1 antibody images:
Western blot - TAP1 antibody (ab52781)
All lanes : Anti-TAP1 antibody (ab52781) at 1/1000 dilution
Lane 1 : a total protein extract from E coli with
50ng to 100 ng of a Tagged irrelevant antigen (Negative control)
Lane 2 : a total protein extract from E coli with
50ng to 500ng of the antigen (Tagged fusion protein)
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase at 1/5000 dilution
Predicted band size : 81 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Note, the molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is fused to GST.
References for Anti-TAP1 antibody (ab52781)
ab52781 has not yet been referenced specifically in any publications.
Publishing research using ab52781? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"