Anti-TARDBP antibody (ab41881)

Western blot - TARDBP antibody (ab41881)

All lanes : Anti-TARDBP antibody (ab41881) at 1 µg/ml

Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 8 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate - tumor cell line (ab29309)

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 45 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)

Immunocytochemistry/ Immunofluorescence - TARDBP antibody (ab41881)

ICC/IF image of ab41881 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41881, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation - TARDBP antibody (ab41881)

Lane 1: Marker, Lane 2: 293T Total Lysate (Input), Lane 3: 293T supernatant (Flow through), LAne 4: Pull down (IP).

This image is courtesy of an Abreview submitted by Dr Jayarama Krishnan Bose

Immunohistochemistry (PFA perfusion fixed frozen sections) - TARDBP antibody (ab41881)

ab41881 staining TARDBP in murine spinal cord by Immunohistochemistry (PFA perfusion fixed frozen sections).

Tissues fixed by 4% paraformaldehyde in phosphate buffer (pH 7.4) were cryoprotected by 30% sucrose in PBS (pH 7.4). Frozen sections (10 µm) of spinal cord tissues from three or more transgenic mice, respectively, were processed for immunofluorescence using ab41881. An Alexa Fluor 594 was used as secondary antibodies to visualize protein. Scale bar 20 µm.

TARDBP is localized in the nucleus and forms intranuclear granular structures, arrowheads.

Image from Shan X et al, Proc Natl Acad Sci U S A. 2010 Sep 14;107(37):16325-30. Epub 2010 Aug 24, Fig 2. DOI 10.1073/pnas.1003459107