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Western blot - TARDBP antibody (ab41881)
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Immunocytochemistry/ Immunofluorescence - TARDBP antibody (ab41881)
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Immunoprecipitation - TARDBP antibody (ab41881)
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Product Name
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TARDBP antibody
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See all TARDBP antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TARDBP
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human TARDBP. (Peptide available as ab41970.)
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IP: 1/50.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following human lysates:
HeLa Whole Cell, HeLa Nuclear, Jurkat Whole Cell, Jurkat Nuclear, HepG2 Whole Cell, HepG2 Nuclear, A431 Whole Cell and K562 Nuclear - tumor cell line.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Neuroscience >> Neurology process >> Neurodegenerative disease >> Amyotrophic lateral sclerosis
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
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Relevance
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HIV1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV1 transcription. In addition, this protein regulates alternate splicing of the CFTR (Cystic Fibrosis Transmembrane Regulator) gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.10 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TARDBP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TARDBP antibody images, references, products related to ab41881 and other tools.
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TARDBP antibody images:
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 Immunocytochemistry/ Immunofluorescence - TARDBP antibody (ab41881)
ICC/IF image of ab41881 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41881, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Immunoprecipitation - TARDBP antibody (ab41881)
Lane 1: Marker,
Lane 2: 293T Total Lysate (Input),
Lane 3: 293T supernatant (Flow through),
LAne 4: Pull down (IP). This image is courtesy of an Abreview submitted by Dr Jayarama Krishnan Bose
See Abreview
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Search PubMed (MEDLINE) for references to TARDBP
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TARDBP antibody - more information
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Customer reviews (feedback) regarding TARDBP antibody |
Customer FAQs regarding TARDBP antibody |
Protocols for TARDBP antibody |
Price and availability of products related to TARDBP antibody |
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TARDBP antibody - related products:
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TARDBP antibody - other tools:
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Contact Abcam with a Technical Enquiry about TARDBP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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