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Products:Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
Overview
Product name
Anti-TARDBP antibody
See all TARDBP products (10) ...
Description
Rabbit polyclonal to TARDBP
Tested applications
IHC-Fr, ICC/IF, WBmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Chicken, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TARDBP.
(Peptide available as ab41971.)
Positive control
This antibody gave a positive signal in the following human lysates: HeLa Whole Cell, HeLa Nuclear, Jurkat Whole Cell, Jurkat Nuclear, HepG2 Whole Cell, HepG2 Nuclear, A431 Whole Cell and K562 Nuclear - tumor cell line.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Neuroscience >> Neurology process >> Neurodegenerative disease >> Amyotrophic lateral sclerosis
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-Fr: 1/50 - 1/200 (See Abreview).
WB: 1/250. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
Tissue specificity
Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
Involvement in disease
Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains.
Domain
The RRM domains can bind to both DNA and RNA.
Post-translational
modifications
Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cellular localization
Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.
Target information above from: UniProt accessionQ13148
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- ALS10 antibody
- OTTHUMP00000002171 antibody
- OTTHUMP00000002172 antibody
see all
Database links
- Entrez Gene: 419453 Chicken
- Entrez Gene: 23435 Human
- Entrez Gene: 230908 Mouse
- Entrez Gene: 298648 Rat
- Omim: 605078 Human
- SwissProt: Q5ZLN5 Chicken
- SwissProt: Q13148 Human
- SwissProt: Q921F2 Mouse
see all
Anti-TARDBP antibody images:
Western blot - TARDBP antibody (ab41972)
All lanes : Anti-TARDBP antibody (ab41972) at 1/250 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Nuclear Lysate (ab14844)
Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 6 : HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)
Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 8 : K562 (Human erythromyeloblastoid leukemia cell line) Nuclear Lysate - tumor cell line (ab29309)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 45 kDa
Observed band size : 47 kDa (why is the actual band size different from the predicted?)
Additional bands at : 26 kDa,28 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - TARDBP antibody (ab41972)
ICC/IF image of ab41972 stained human Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab41972, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-TARDBP antibody (ab41972)
ab41972 has not yet been referenced specifically in any publications.
Publishing research using ab41972? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"