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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab42474 for help.
There are no answered questions for ab42474
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - TARDBP antibody (ab42474)
All lanes : Anti-TARDBP antibody (ab42474) at 1 µg/ml
Lane 1 :
Lane 2 :
Lane 3 :
Lane 4 : Brain (Mouse) Tissue Lysate
Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 6 : Spinal Cord (Mouse) Tissue Lysate
Lane 7 : Brain (Rat) Tissue Lysate - normal tissue
Lane 8 : Rat DRG
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 45 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - TARDBP antibody (ab42474)
ICC/IF image of ab42474 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab42474, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.
TARDBP antibody for IHC-P in Rat (42474)
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