Anti-TARDBP antibody (ab50930)

Overview

• Product nameAnti-TARDBP antibodySee all TARDBP primary antibodies ...
• Description
  Rabbit polyclonal to TARDBP
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog
• Immunogen

  A region within synthetic peptide: MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC, corresponding to N terminal amino acids 1-50 of Human TARDBP

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• Positive controlHepG2 cell lysate, Human liver and kidney tubules.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Microbiology
  • Organism
  • Virus
  • RNA Virus
  • ssRNA positive strand virus
  • HIV

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Splicing

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Amyotrophic lateral sclerosis

Applications

Target

• FunctionDNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR.
• Tissue specificityUbiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen.
• Involvement in diseaseDefects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases.
• Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
• DomainThe RRM domains can bind to both DNA and RNA.
• Post-translational
  modificationsHyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
  Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
  Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
• Cellular localizationNucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies.

Target information above from: UniProt accession Q13148 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 23435 Human
  • Entrez Gene: 230908 Mouse
  • Entrez Gene: 298648 Rat
  • Omim: 605078 Human
  • SwissProt: Q13148 Human
  • SwissProt: Q921F2 Mouse
  • Unigene: 300624 Human
  • Unigene: 635053 Human

  • Unigene: 22453 Mouse
  • Unigene: 2633 Rat

  see all

• Alternative names
  ALS10 antibodyOTTHUMP00000002171 antibodyOTTHUMP00000002172 antibody

  OTTHUMP00000002173 antibodyTADBP_HUMAN antibodyTAR DNA binding protein 43 antibodyTAR DNA binding protein antibodyTAR DNA-binding protein 43 antibodyTARDBP antibodyTDP 43 antibodyTDP-43 antibodyTDP43 antibody

  see all

Anti-TARDBP antibody images

• 

  Western blot - TARDBP antibody (ab50930)
  Anti-TARDBP antibody (ab50930) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 45 kDa
  Observed band size : 45 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
  Ab50930 at 4.0 µg/ml staining epithelial cells of renal tubule from Human kidney; paraffin embedded, magnification 400x.
• 

  Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
  Ab50930 at 4.0 µg/ml staining Human liver hepatocytes; paraffin embedded, magnification 400x.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TARDBP antibody(ab50930)
  Immunohistochemistry of Human Liver cell lysate tissue at an antibody concentration of 4-8µg/ml using anti-TARDBP antibody (ab50930)
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TARDBP antibody(ab50930)
  Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-TARDBP antibody (ab50930)