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Western blot - TARDBP antibody (ab50930)
Western blot - TARDBP antibody (ab50930)
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Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
(enlarge)


Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
(enlarge)


Product Name 

TARDBP antibody

 

See all TARDBP antibodies (8)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to TARDBP

Immunogen 

A region within synthetic peptide: MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC, corresponding to N terminal amino acids 1-50 of Human TARDBP
BLAST 'MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC ' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: Titre using peptide based assay 1/1562500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HepG2 cell lysate, Human liver and kidney tubules.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Neuroscience >> Neurology process >> Neurodegenerative disease >> Amyotrophic lateral sclerosis
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV

Relevance 

HIV1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV1 transcription. In addition, this protein regulates alternate splicing of the CFTR (Cystic Fibrosis Transmembrane Regulator) gene.

 

Alternative names TARDBP antibody (ab50930)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

23435    (Human)

Omim

    

605078    (Human)

SwissProt

    

Q13148    (Human)

Unigene

    

300624    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: None
Constituents: 2% Sucrose, PBS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TARDBP antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for TARDBP antibody images, references, products related to ab50930 and other tools.

 

TARDBP antibody images:

Product image  Western blot - TARDBP antibody (ab50930)

Western blot - TARDBP antibody (ab50930)

TARDBP antibody (ab50930) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 45 kDa
Observed band size : 45 kDa


Diluted in 5% skim milk / PBS buffer, gel concentration 12%

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)

Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)

Ab50930 at 4.0 µg/ml staining epithelial cells of renal tubule from Human kidney; paraffin embedded, magnification 400x.

 

Product image  Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)

Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)

Ab50930 at 4.0 µg/ml staining Human liver hepatocytes; paraffin embedded, magnification 400x.

 
 

Search PubMed (MEDLINE) for references to TARDBP

 

TARDBP antibody - more information

Customer reviews (feedback) regarding TARDBP antibody

Customer FAQs regarding TARDBP antibody

Protocols for TARDBP antibody

Price and availability of products related to TARDBP antibody

 

TARDBP antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for TARDBP antibody

ab14660

  

HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate

ab4347

  

Kidney (human) normal tissue slides

ab4348

  

Liver (human) normal tissue slides

 

Compatible Secondaries for TARDBP antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other TARDBP Antibodies

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TARDBP antibody - other tools:

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