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Western blot - TARDBP antibody (ab50930)
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Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
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Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
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Product Name
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TARDBP antibody
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See all TARDBP antibodies (8)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TARDBP
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Immunogen
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A region within synthetic peptide: MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC, corresponding to N terminal amino acids 1-50 of Human TARDBP
BLAST 'MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Titre using peptide based assay 1/1562500.
IHC-P: Use at a concentration of 4 - 8 µg/ml.
WB: Use at a concentration of 1.25 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HepG2 cell lysate, Human liver and kidney tubules.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurodegenerative disease >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing
Neuroscience >> Neurology process >> Neurodegenerative disease >> Amyotrophic lateral sclerosis
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
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Relevance
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HIV1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV1 transcription. In addition, this protein regulates alternate splicing of the CFTR (Cystic Fibrosis Transmembrane Regulator) gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TARDBP antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TARDBP antibody images, references, products related to ab50930 and other tools.
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TARDBP antibody images:
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 Western blot - TARDBP antibody (ab50930)
TARDBP antibody (ab50930) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 45 kDa
Observed band size : 45 kDa
Diluted in 5% skim milk / PBS buffer, gel concentration 12%
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Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
Ab50930 at 4.0 µg/ml staining epithelial cells of renal tubule from Human kidney; paraffin embedded, magnification 400x.
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Immunohistochemistry (Paraffin-embedded sections) - TARDBP antibody (ab50930)
Ab50930 at 4.0 µg/ml staining Human liver hepatocytes; paraffin embedded, magnification 400x.
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Search PubMed (MEDLINE) for references to TARDBP
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TARDBP antibody - more information
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Customer reviews (feedback) regarding TARDBP antibody |
Customer FAQs regarding TARDBP antibody |
Protocols for TARDBP antibody |
Price and availability of products related to TARDBP antibody |
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TARDBP antibody - related products:
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TARDBP antibody - other tools:
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Contact Abcam with a Technical Enquiry about TARDBP antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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