Anti-TATA binding protein TBP antibody [58C9] (ab61411)

Overview

• Product nameAnti-TATA binding protein TBP antibody [58C9]See all TATA binding protein TBP primary antibodies ...
• Description
  Mouse monoclonal [58C9] to TATA binding protein TBP
• Specificityab61411 recognises TATA binding protein TBP also in Sf9 cells.
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Human, Saccharomyces cerevisiae, Fruit fly (Drosophila melanogaster), Corn
  
• Immunogen

  Drosophila TFIID complex.

• Positive controlNuclear extracts of D melanogaster cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 15mM Sodium Azide
  Constituents: 0.01M PBS, pH 7.4
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone number58C9
• IsotypeIgG2b
• Research Areas
  • Isotype/Loading Controls
  • Loading Controls
  • TBP - Nuclear

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TAFs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TFIIB

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Preinitiation complex

Applications

Target

• FunctionGeneral transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
• Tissue specificityWidely expressed, with levels highest in the testis and ovary.
• Involvement in diseaseDefects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
• Sequence similaritiesBelongs to the TBP family.
• Cellular localizationNucleus.

Target information above from: UniProt accession P20226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 37476 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 6908 Human
  • Omim: 600075 Human
  • SwissProt: P20227 Fruit fly (Drosophila melanogaster)
  • SwissProt: P20226 Human
  • Unigene: 4490 Fruit fly (Drosophila melanogaster)
  • Unigene: 1100 Human
  • Unigene: 590872 Human

• Alternative names
  GTF2D antibodyGTF2D1 antibodyHDL4 antibody

  MGC117320 antibodyMGC126054 antibodyMGC126055 antibodySCA17 antibodyTATA binding factor antibodyTATA box factor antibodyTATA sequence binding protein antibodyTATA sequence-binding protein antibodyTATA-binding factor antibodyTATA-box binding protein N-terminal domain antibodyTATA-box factor antibodyTATA-box-binding protein antibodyTBP antibodyTBP_HUMAN antibodyTFIID antibodyTranscription initiation factor TFIID TBP subunit antibody

  see all

Anti-TATA binding protein TBP antibody [58C9] images

• 

  Western blot - TATA binding protein TBP antibody [58C9] (ab61411)
  Lane 1 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 2 µg/ml
  Lane 2 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 1 µg/ml
  Lane 3 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 0.5 µg/ml
  Lane 4 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) at 0.2 µg/ml
  Lane 5 : Anti-TATA binding protein TBP antibody [58C9] (ab61411) (Negative control )
  
  Lane 1 : Nuclear cell extract of D.Mel cells
  Lane 2 : Nuclear cell extract of D.Mel cells
  Lane 3 : Nuclear cell extract of D.Mel cells
  Lane 4 : Nuclear cell extract of D.Mel cells
  Lane 5 : Nuclear cell extract of D.Mel cells
  
  Lysates/proteins at 20 µg/ml per lane.
  
  Secondary
  Goat Anti-Mouse – Peroxidase conjugate
  developed using the ECL technique
  
  Predicted band size : 38 kDa
  Observed band size : 39 kDa (why is the actual band size different from the predicted?)