Anti-TATA binding protein TBP antibody (ab23474)

Overview

• Product nameAnti-TATA binding protein TBP antibodySee all TATA binding protein TBP primary antibodies ...
• Description
  Rabbit polyclonal to TATA binding protein TBP
• Specificityab23474 reacts with transcription initiation factor TATA binding protein TBP subunit 7 (TAF7).
• Tested applicationsWB, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog, Zebrafish
• Immunogen

  A region within a synthetic peptide: MSKSKDDAPH ELESQFILRL PPEYASTVRR AVQSGHVNLK DRLTIELHPD, corresponding to N terminal amino acids 1-50 of Human TATA binding protein TBP

  Run BLAST with Run BLAST with
• Positive controlJurkat cell lysate, human lung and human kidney.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityIgG fraction
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Isotype/Loading Controls
  • Loading Controls
  • TBP - Nuclear

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TFIIB

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TAFs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Preinitiation complex

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TFIID

Applications

Target

• FunctionGeneral transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
• Tissue specificityWidely expressed, with levels highest in the testis and ovary.
• Involvement in diseaseDefects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
• Sequence similaritiesBelongs to the TBP family.
• Cellular localizationNucleus.

Target information above from: UniProt accession P20226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 431689 Dog
  • Entrez Gene: 6908 Human
  • Entrez Gene: 21374 Mouse
  • Entrez Gene: 117526 Rat
  • Entrez Gene: 368882 Zebrafish
  • Omim: 600075 Human
  • SwissProt: P20226 Human
  • SwissProt: P29037 Mouse

  • SwissProt: Q71H62 Zebrafish
  • SwissProt: Q7SXL3 Zebrafish
  • Unigene: 1100 Human
  • Unigene: 590872 Human
  • Unigene: 244820 Mouse
  • Unigene: 22712 Rat
  • Unigene: 117556 Zebrafish

  see all

• Alternative names
  GTF2D antibodyGTF2D1 antibodyHDL4 antibody

  MGC117320 antibodyMGC126054 antibodyMGC126055 antibodySCA17 antibodySpinocerebellar ataxia 17 antibodyTATA binding factor antibodyTATA box factor antibodyTATA sequence binding protein antibodyTATA sequence-binding protein antibodyTATA-binding factor antibodyTATA-box binding protein N-terminal domain antibodyTATA-box factor antibodyTATA-box-binding protein antibodyTBP antibodyTBP_HUMAN antibodyTFIID antibodyTranscription initiation factor TFIID TBP subunit antibody

  see all

Anti-TATA binding protein TBP antibody images

• 

  Western blot - TATA binding protein TBP antibody (ab23474)
  Anti-TATA binding protein TBP antibody (ab23474) at 2 µg/ml + Jurkat cell lysate
  
  Secondary
  HRP conjugated anti-Rabbit IgG
  
  Performed under reducing conditions.
  
  Predicted band size : 38 kDa
  Observed band size : 38 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - TATA binding protein TBP antibody (ab23474)
  Cells with positive label: Epithelial cells of renal tubule (indicated with arrows). Antibody concentration 4-8ug/ml. Magnification 400X.
• 

  Immunohistochemistry (Paraffin-embedded sections) - TATA binding protein TBP antibody (ab23474)
  Cells with positive label: Epithelial cells of bronchiole (indicated with arrows). Antibody concentration 4-8ug/ml. Magnification 400X.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TATA binding protein TBP antibody(ab23474)
  Immunohistochemistry of Human Lung lysate tissue at an antibody concentration of 4-8µg/ml using anti-TAF7 antibody (ab23474)
  
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-TATA binding protein TBP antibody(ab23474)
  Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-TAF7 antibody (ab23474)