Anti-TATA binding protein TBP antibody (ab52701)

Overview

• Product nameAnti-TATA binding protein TBP antibodySee all TATA binding protein TBP primary antibodies ...
• Description
  Mouse polyclonal to TATA binding protein TBP
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Plasmodium falciparum
  
• Immunogen

  Recombinant fusion protein: RNAEYNPSKI NTLIIRLNKP QCTALIFKNG RIMLTGTRTK KDSIMGCKKI AKIIKIVTKD KVKFCNFKIE NIIASANCNI PIRLEVLAHD HKEYCNYEPE , corresponding to amino acids 75-174 of Plasmodium falciparum TATA binding protein TBP

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Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 50% Glycerol, Whole serum
• PurityWhole antiserum
• Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Isotype/Loading Controls
  • Loading Controls
  • TBP - Nuclear

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TAFs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • TFIIB

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Preinitiation complex

Applications

Target

• FunctionGeneral transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
• Tissue specificityWidely expressed, with levels highest in the testis and ovary.
• Involvement in diseaseDefects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
• Sequence similaritiesBelongs to the TBP family.
• Cellular localizationNucleus.

Target information above from: UniProt accession P20226 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

• Alternative names
  GTF2D antibodyGTF2D1 antibodyHDL4 antibody

  MGC117320 antibodyMGC126054 antibodyMGC126055 antibodySCA17 antibodyTATA binding factor antibodyTATA box factor antibodyTATA sequence binding protein antibodyTATA sequence-binding protein antibodyTATA-binding factor antibodyTATA-box binding protein N-terminal domain antibodyTATA-box factor antibodyTATA-box-binding protein antibodyTBP antibodyTBP_HUMAN antibodyTFIID antibodyTranscription initiation factor TFIID TBP subunit antibody

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Anti-TATA binding protein TBP antibody images

• 

  Western blot - TATA binding protein TBP antibody (ab52701)
  All lanes : Anti-TATA binding protein TBP antibody (ab52701) at 1/1000 dilution
  
  Lane 1 : ~20µg of a total protein extract from E coli with ~50ng to 100ng of a fusion protein of an irrelevant antigen
  Lane 2 : ~20µg of a total protein extract from E coli with ~50ng to 500ng of the antigen (antigen fusion protein)
  
  Secondary
  Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
  
  Predicted band size : 38 kDa
  Observed band size : 25 kDa (why is the actual band size different from the predicted?)