Anti-TATA binding protein TBP antibody (ab52701)

Overview

  • Product nameAnti-TATA binding protein TBP antibodySee all TATA binding protein TBP primary antibodies ...
  • Description
    Mouse polyclonal to TATA binding protein TBP
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Plasmodium falciparum
  • Immunogen

    Recombinant fusion protein: RNAEYNPSKI NTLIIRLNKP QCTALIFKNG RIMLTGTRTK KDSIMGCKKI AKIIKIVTKD KVKFCNFKIE NIIASANCNI PIRLEVLAHD HKEYCNYEPE , corresponding to amino acids 75-174 of Plasmodium falciparum TATA binding protein TBP

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab52701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: 1/1000. Detects a band of approximately 25 kDa for the fusion protein (predicted molecular weight for the full length protein: 38 kDa).
    This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionGeneral transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
    • Tissue specificityWidely expressed, with levels highest in the testis and ovary.
    • Involvement in diseaseDefects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
    • Sequence similaritiesBelongs to the TBP family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Alternative names
      • GTF2D antibody
      • GTF2D1 antibody
      • HDL4 antibody
      • MGC117320 antibody
      • MGC126054 antibody
      • MGC126055 antibody
      • SCA17 antibody
      • TATA binding factor antibody
      • TATA box factor antibody
      • TATA sequence binding protein antibody
      • TATA sequence-binding protein antibody
      • TATA-binding factor antibody
      • TATA-box binding protein N-terminal domain antibody
      • TATA-box factor antibody
      • TATA-box-binding protein antibody
      • TBP antibody
      • TBP_HUMAN antibody
      • TFIID antibody
      • Transcription initiation factor TFIID TBP subunit antibody
      see all

    Anti-TATA binding protein TBP antibody images

    • All lanes : Anti-TATA binding protein TBP antibody (ab52701) at 1/1000 dilution

      Lane 1 : ~20µg of a total protein extract from E coli with ~50ng to 100ng of a fusion protein of an irrelevant antigen
      Lane 2 : ~20µg of a total protein extract from E coli with ~50ng to 500ng of the antigen (antigen fusion protein)

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size : 38 kDa
      Observed band size : 25 kDa (why is the actual band size different from the predicted?)

    References for Anti-TATA binding protein TBP antibody (ab52701)

    This product has been referenced in:
    • Huang G  et al. NF-?B plays a key role in inducing CD274 expression in human monocytes after lipopolysaccharide treatment. PLoS One 8:e61602 (2013). WB ; Human . Read more (PubMed: 23585913) »

    See 1 Publication for this product

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    We are not currently aware of any publications which have used this product in plasmodium berghei. However as these sequences are well matched, this product would qualify for enrollment in our 100% Abreview Tes...

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