The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. These proteins are responsible for the high affinity of mycobacteria to fibronectin. All three proteins possesses a mycolyltransferase activity - which is required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Thus all three proteins are involved in the final stages of mycobacterial cell wall assembly.
Secreted; cell wall. Cytoplasm
85 A antibody
Antigen 85 A antibody
Antigen 85 complex A antibody
Antigen 85A antibody
Fbp A antibody
Fibronectin binding protein A antibody
Mpt 44 antibody
Mycobacterium tuberculosis antigen 85A antibody
Mycolyl transferase 85 A antibody
Mycolyl transferase 85A antibody
Secreted antigen Ag85A antibody
Anti-TB Ag85A antibody (FITC) images
Western blot - Anti-TB Ag85A antibody (FITC) (ab193480)
All lanes : Anti-TB Ag85A antibody (FITC) (ab193480) at 2 µg/ml
Lane 1 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 1 µg Lane 2 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 10 µg
Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size : 36 kDa Observed band size : 36 kDa