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Product name
Anti-TBC1D4 antibody
See all TBC1D4 products (10) ...
Description
Rabbit polyclonal to TBC1D4
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Dog
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1250 to the C-terminus of Human TBC1D4.
(Peptide available as ab263 27.)
Positive control
This antibody gave a positive signal in human skeletal muscle tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 147 kDa).
IP: Use at an assay dependent dilution. PubMed: 20937822
IHC-P: 1/135. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Tissue specificity
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T cells from patients with atopic dermatitis.
Sequence similarities
Contains 2 PID domains.
Contains 1 Rab-GAP TBC domain.
Post-translational
modifications
Phosphorylated by AKT1; insulin-induced.
Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation.
Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Cellular localization
Cytoplasm. Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
Target information above from: UniProt accessionO60343
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Acrg embryonic lethality (mouse) minimal region ortholog antibody
- Acrg embryonic lethality minimal region ortholog antibody
- Acrg embryonic lethality mouse minimal region ortholog antibody
see all
Database links
- Entrez Gene: 9882 Human
- Omim: 612465 Human
- SwissProt: O60343 Human
- Unigene: 173802 Human
- Unigene: 210891 Human
Anti-TBC1D4 antibody images:
Western blot - TBC1D4 antibody (ab24469)
All lanes : Anti-TBC1D4 antibody (ab24469) at 0.46 µg/ml
Lane 1 : Human skeletal muscle cell lysate at 10 µg
Lane 2 : Human skeletal muscle cell lysate at 20 µg
Lane 3 : Human skeletal muscle cell lysate at 40 µg
Lane 4 : Human skeletal muscle cell lysate at 60 µg
Secondary
Anti-rabbit HRP
Predicted band size : 147 kDa
Observed band size : 160 kDa (why is the actual band size different from the predicted?)
Incubation with the primary antibody was at 4 degrees celsius overnight. The secondary anti-rabbit HRP antibody was incubated for one hour at room temperature.
Jonas Treebak
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TBC1D4 antibody (ab24469)
Image courtesy of Human Protein Atlas
ab24469 staining TBC1D4 in human stomach. Paraffin embedded human stomach tissue was incubated with ab24469 (1/135 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab24469 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
Western blot - TBC1D4 antibody (ab24469)
Anti-TBC1D4 antibody (ab24469) at 1 µg/ml + TCB1D4 Human Partial Recombinant Protein (His Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 147 kDa
Observed band size : 15 kDa (why is the actual band size different from the predicted?)
References for Anti-TBC1D4 antibody (ab24469)
This product has been referenced in:
- Martin JS et al. Enhanced external counterpulsation improves peripheral artery function and glucose tolerance in subjects with abnormal glucose tolerance. J Appl Physiol 112:868-76 (2012). WB; Human.Read more (PubMed: 22194326) »
- Mendes AI et al. Protein Kinase WNK1 Promotes Cell Surface Expression of Glucose Transporter GLUT1 by Regulating a Tre-2/USP6-BUB2-Cdc16 Domain Family Member 4 (TBC1D4)-Rab8A Complex. J Biol Chem 285:39117-26 (2010). WB, IP; Human.Read more (PubMed: 20937822) »
See all 6 publications for this product
Publishing research using ab24469? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"