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Product name
Anti-TBL1 antibody - ChIP Grade
See all TBL1 products (4) ...
Description
Rabbit polyclonal to TBL1 - ChIP Grade
Specificity
Does not cross-react with TBLR1.
Tested applications
ChIP, WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human)
Positive control
HeLa nuclear extract
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Neuroscience >> Sensory System >> Auditory system
Applications
Show applications key
Our Abpromise guarantee covers the use of ab24548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 63 kDa).
IP: Use at an assay dependent dilution.
ICC/IF: 1/300.
Target
Function
F-box-like protein involved in the recruitment of the ubiquitin/19S proteasome complex to nuclear receptor-regulated transcription units. Plays an essential role in transcription activation mediated by nuclear receptors. Probably acts as integral component of corepressor complexes that mediates the recruitment of the 19S proteasome complex, leading to the subsequent proteasomal degradation of transcription repressor complexes, thereby allowing cofactor exchange.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the WD repeat EBI family.
Contains 1 F-box-like domain.
Contains 1 LisH domain.
Contains 8 WD repeats.
Domain
The F-box-like domain is related to the F-box domain, and apparently displays the same function as component of ubiquitin E3 ligase complexes.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO60907
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- X-linked antibody
- EBI antibody
- F box like/WD repeat protein TBL1X antibody
see all
Database links
- Entrez Gene: 6907 Human
- Omim: 300196 Human
- SwissProt: O60907 Human
- Unigene: 495656 Human
Anti-TBL1 antibody - ChIP Grade images:
Western blot - TBL1 antibody - ChIP Grade (ab24548)
Anti-TBL1 antibody - ChIP Grade (ab24548) at 1/1000 dilution + HeLa nuclear extract.
Predicted band size : 63 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)
Immunoprecipitation - Anti-TBL1 antibody - ChIP Grade (ab24548)
TBL1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to TBL1 - ChIP Grade (ab24548) and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab24548.
Secondary: Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 64kDa: TBL1.
Immunocytochemistry/ Immunofluorescence - TBL1 antibody - ChIP Grade (ab24548)
ab24548 staining TBL1 (green) in Human Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 0.2% fish scale gelatin) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
References for Anti-TBL1 antibody - ChIP Grade (ab24548)
This product has been referenced in:
- Ramadoss S et al. Transducin ß-like protein 1 recruits nuclear factor ?B to the target gene promoter for transcriptional activation. Mol Cell Biol 31:924-34 (2011). ChIP.Read more (PubMed: 21189284) »
- Dimitrova YN et al. Direct ubiquitination of beta-catenin by Siah-1 and regulation by the exchange factor TBL1. J Biol Chem 285:13507-16 (2010). WB; Human.Read more (PubMed: 20181957) »
See all 4 publications for this product
Publishing research using ab24548? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"