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Rabbit polyclonal to TBX5
Recombinant fragment within Zebrafish TBX5 aa 20-262. The exact sequence is proprietary.
Database link: Q9IAK8
Zebrafish eye whole cell lysate; Zebrafish tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Suggested antigen retrieval using 1mM EDTA-NaOH (pH8.0) at 95°C,1Hr
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
Involved in the transcriptional regulation of genes required for mesoderm differentiation. Probably plays a role in limb pattern formation.
Involvement in disease
Defects in TBX5 are the cause of Holt-Oram syndrome (HOS) [MIM:142900]. HOS is a developmental disorder affecting the heart and upper limbs. It is characterized by thumb anomaly and atrial septal defects.
Contains 1 T-box DNA-binding domain.
Information by UniProt
Holt Oram syndrome antibody
T box 5 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBX5 antibody (ab210111)
Immunohistochemical analysis of paraffin-embedded Zebrafish tissue labeling TBX5 with ab210111 at 1/300 dilution.
Western blot - Anti-TBX5 antibody (ab210111)
Anti-TBX5 antibody (ab210111) at 1/500 dilution + Zebrafish eye whole cell lysate at 30 µg
Predicted band size :
10% SDS PAGE
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"