Recombinant
RabMAb

Anti-TCEA1 antibody [EPR14821] (ab185947)

Overview

  • Product name
    Anti-TCEA1 antibody [EPR14821]
    See all TCEA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14821] to TCEA1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TCEA1 aa 200-300. The exact sequence is proprietary.
    Database link: Q29RL9

  • Positive control
    • K562, HeLa, SP2/0 and HUVEC cell lysate; Human bladder transitional cell carcinoma tissue; HeLa, HUVEC cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab185947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/500.
ICC/IF 1/500.
Flow Cyt 1/250. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/30 - 1/50.

Target

  • Function
    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • Involvement in disease
    Note=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similarities
    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTF2S antibody
    • SII antibody
    • TCEA antibody
    • Tcea1 antibody
    • TCEA1_HUMAN antibody
    • TF2S antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 1 antibody
    • Transcription elongation factor A protein 1 antibody
    • Transcription elongation factor A, 1 antibody
    • Transcription elongation factor S-II protein 1 antibody
    • Transcription elongation factor TFIIS.o antibody
    see all

Images

  • All lanes : Anti-TCEA1 antibody [EPR14821] (ab185947) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SP2/0 cell lysate
    Lane 4 : HUVEC cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 34 kDa
  • Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling TCEA1 with ab185947 at 1/500 dilution. Counterstained with Hematoxylin.

  • Immunofluorescent staining of -20 °C acetone fixed HeLa cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and  DAPI nuclear staining (blue; right image).  Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.

  • Immunofluorescent staining of 4% paraformaldehyde fixed HUVEC cells labeling TCEA1 with ab185947 at 1/500 dilution (red; left image) and  DAPI nuclear staining (blue; right image).  Secondary staining with Goat anti Rabbit IgG (Alexa Fluor® 555) at a 1/200 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde HeLa cells labeling TCEA1 with ab185947 at 1/250 dilution (red) compared to a Rabbit monoclonal IgG isotype control antibody (green).  Secondary staining with Goat anti Rabbit IgG (FITC) at a 1/150 dilution.

  • Immunoprecipitation analysis of HeLa cell lysate labeling TCEA1 with ab185947 at 1/50 dilution.  Secondary staining with Goat anti Rabbit IgG (H+L) Peroxidase conjugated antibody at a 1/1000 dilution.

References

ab185947 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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