Recombinant
RabMAb

Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

Overview

  • Product name
    Anti-TCEA1 antibody [EPR15036-16] - N-terminal
    See all TCEA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR15036-16] to TCEA1 - N-terminal
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human TCEA1 aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: P23193

  • Positive control
    • Jurkat, K562, 293 and HUVEC lysates. HUVEC cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab184159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 34, 38 kDa (predicted molecular weight: 34 kDa).
IP 1/60 - 1/80.
ICC/IF 1/250.

Target

  • Function
    Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
  • Involvement in disease
    Note=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
  • Sequence similarities
    Belongs to the TFS-II family.
    Contains 1 TFIIS central domain.
    Contains 1 TFIIS N-terminal domain.
    Contains 1 TFIIS-type zinc finger.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • GTF2S antibody
    • SII antibody
    • TCEA antibody
    • Tcea1 antibody
    • TCEA1_HUMAN antibody
    • TF2S antibody
    • TFIIS antibody
    • Transcription elongation factor A (SII) 1 antibody
    • Transcription elongation factor A protein 1 antibody
    • Transcription elongation factor A, 1 antibody
    • Transcription elongation factor S-II protein 1 antibody
    • Transcription elongation factor TFIIS.o antibody
    see all

Anti-TCEA1 antibody [EPR15036-16] - N-terminal images

  • Immunofluorscence analysis of HUVEC cells labeling TCEA1 with ab184159 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution (left). Counter-stained with Dapi (right). 

  • Immunoprecipitation of Jurkat lysate labelling TCEA1 with ab184159 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody used at 1/1000 dilution

  • All lanes : Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159) at 1/50000 dilution

    Lane 1 : Jurkat lysate
    Lane 2 : K562 lysate
    Lane 3 : 293 lysate
    Lane 4 : HUVEC lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 34 kDa

References for Anti-TCEA1 antibody [EPR15036-16] - N-terminal (ab184159)

ab184159 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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