The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 34, 38 kDa (predicted molecular weight: 34 kDa).
1/60 - 1/80.
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Involvement in disease
Note=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) . Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
Belongs to the TFS-II family. Contains 1 TFIIS central domain. Contains 1 TFIIS N-terminal domain. Contains 1 TFIIS-type zinc finger.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Immunofluorscence analysis of HUVEC cells labeling TCEA1 with ab184159 at 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution (left). Counter-stained with Dapi (right).