Anti-TCEA1 antibody (ab76637)

Overview

• Product nameAnti-TCEA1 antibodySee all TCEA1 primary antibodies ...
• Description
  Mouse monoclonal to TCEA1
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Recombinant fragment, corresponding to amino acids 81-185 of Human TCEA1 with tag (NP_006747).

• Positive controlTCEA1 transfected 293T cell lysate; Recombinant fragment of human TCEA1.

Properties

• FormLiquid
• Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Elongation factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • Other

Applications

Target

• FunctionNecessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
• Involvement in diseaseNote=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.
• Sequence similaritiesBelongs to the TFS-II family.
  Contains 1 TFIIS central domain.
  Contains 1 TFIIS N-terminal domain.
  Contains 1 TFIIS-type zinc finger.
• Post-translational
  modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationNucleus.

Target information above from: UniProt accession P23193 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6917 Human
  • Omim: 601425 Human
  • SwissProt: P23193 Human
  • Unigene: 491745 Human

• Alternative names
  GTF2S antibodySII antibodyTCEA antibody

  Tcea1 antibodyTCEA1_HUMAN antibodyTF2S antibodyTFIIS antibodyTranscription elongation factor A (SII) 1 antibodyTranscription elongation factor A protein 1 antibodyTranscription elongation factor S-II protein 1 antibodyTranscription elongation factor TFIIS.o antibody

  see all

Anti-TCEA1 antibody images

• 

  Western blot - TCEA1 antibody (ab76637)
  Anti-TCEA1 antibody (ab76637) at 1/500 dilution + Recombinant fragment of human TCEA1 at 0.2 µg
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
  
  Predicted band size : 34 kDa
  Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - TCEA1 antibody (ab76637)
  All lanes : Anti-TCEA1 antibody (ab76637) at 1/500 dilution
  
  Lane 1 : TCEA1 transfected 293T cell lysate
  Lane 2 : Non-transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
  
  Predicted band size : 34 kDa
  Observed band size : 34 kDa
  
• 

  ELISA - TCEA1 antibody (ab76637)
  The antibody concentration should be 10µg/mL in coating buffer (PBS), the volume should be 100µL/well. Blocking solution was added at 250ul per well (5% Skim Milk in PBS + 0.05% Tween-20). Secondary antibody used was HRP conjugated goat anti-rabbit IgG (H+L).