Anti-TCEAL1 antibody (ab77661)

Overview

• Product nameAnti-TCEAL1 antibodySee all TCEAL1 primary antibodies ...
• Description
  Mouse monoclonal to TCEAL1
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human, Recombinant Fragment
  
• Immunogen

  Recombinant fragment: MDKPRKENEE EPQSAPKTDE ERPPVEHSPE KQSPEEQSSE EQSSEEEFFP EELLPELLPE MLLSEERPPQ EGLSRKDLFE GRPPMEQPPC GVGKHKLEEG , corresponding to amino acids 1-100 of Human TCEAL1 with tag (NP_001006640)

  Run BLAST with Run BLAST with
• Positive controlTCEAL1 transfected 293T cell lysate; Recombinant fragment of human TCEAL1.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Elongation factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol II Transcription
  • Other

Applications

Target

• FunctionMay be involved in transcriptional regulation. Modulates various viral and cellular promoters in a promoter context-dependent manner. For example, transcription from the FOS promoter is increased, while Rous sarcoma virus (RSV) long terminal repeat (LTR) promoter activity is repressed. Does not bind DNA directly.
• Tissue specificityExpressed in all tissues examined. Highly expressed in heart, ovary, prostate and skeletal muscle. Moderately expressed in brain, placenta, testis and small intestine. Weakly expressed in lung, liver and spleen. Expressed in several cancer cell lines.
• Sequence similaritiesBelongs to the TFS-II family. TFA subfamily.
• Post-translational
  modificationsPhosphorylation of Ser-36 and Ser-37 is critical for transcriptional repression.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q15170 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 9338 Human
  • Omim: 300237 Human
  • SwissProt: Q15170 Human
  • Unigene: 95243 Human

• Alternative names
  Highly similar to gene pp21 protein [H.sapiens] antibodyNuclear phosphoprotein p21/SIIR antibodyp21 antibody

  pp21 antibodySIIR antibodyTCAL1_HUMAN antibodyTCEA like protein 1 antibodyTCEA-like protein 1 antibodyTCEAL1 antibodyTranscription elongation factor A (SII) like 1 antibodyTranscription elongation factor A protein like 1 antibodyTranscription elongation factor A protein-like 1 antibodyTranscription elongation factor S II protein like 1 antibodyTranscription elongation factor S-II protein-like 1 antibody

  see all

Anti-TCEAL1 antibody images

• 

  Western blot - TCEAL1 antibody (ab77661)
  Anti-TCEAL1 antibody (ab77661) at 5 µg/ml + Recombinant fragment of human TCEAL1 at 0.2 µg
  
  Secondary
  Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
  
  Predicted band size : 18 kDa
  Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - TCEAL1 antibody (ab77661)
  All lanes : Anti-TCEAL1 antibody (ab77661) at 5 µg/ml
  
  Lane 1 : TCEAL1 transfected 293T cell lysate
  Lane 2 : Non-transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
  
  Predicted band size : 18 kDa
  Observed band size : 24 kDa (why is the actual band size different from the predicted?)