Overview

  • Product name
  • Description
    Rabbit polyclonal to TCEB2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Goat, Cow, Dog, Pig, Chimpanzee, Lizard, Snake, Cynomolgus monkey, Rhesus monkey, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 68-118 of Human TCEB2 (NP_009039.1).

  • Positive control
    • Jurkat, 293T, HeLa, mouse TCMK-1 and mouse NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab168836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Predicted molecular weight: 13 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
    The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Contains 1 ubiquitin-like domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • EloB antibody
    • ELOB_HUMAN antibody
    • Elongin 18 kDa subunit antibody
    • Elongin B antibody
    • Elongin-B antibody
    • RNA polymerase II transcription factor SIII p18 subunit antibody
    • RNA polymerase II transcription factor SIII subunit B antibody
    • SIII antibody
    • SIII p18 antibody
    • TCEB 2 antibody
    • Tceb2 antibody
    • Transcription elongation factor B (SIII) polypeptide 2 antibody
    • Transcription elongation factor B polypeptide 2 antibody
    see all

Images

  • All lanes : Anti-TCEB2 antibody (ab168836) at 1 µg/ml

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 13 kDa


    Exposure time : 3 minutes
  • Detection of TCEB2 in Immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168836 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 3 minutes.

References

This product has been referenced in:
  • Chuang TW  et al. A Point Mutation in the Exon Junction Complex Factor Y14 Disrupts Its Function in mRNA Cap Binding and Translation Enhancement. J Biol Chem 291:8565-74 (2016). Read more (PubMed: 26887951) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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