Overview

  • Product name
  • Description
    Rabbit polyclonal to TCF15
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog
  • Immunogen

    Synthetic peptide: AFALLRPVGA HVLYPDVRLL SEDEENRSES DASDQSFGCC EGPEAARRGP , corresponding to amino acids 2-51 of Human TCF15

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-TCF15 antibody (ab66371) at 2.5 µg/ml + Jurkat cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution

    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

References

ab66371 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab66371.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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